Maturation, Plasticité, Physiologie et Pathologie de la Respiration, Unité Mixte de Recherche 6231, Centre National de la Recherche Scientifique - Université de la Méditerranée - Université Paul Cézanne, Marseille, France.
PLoS One. 2010 Oct 26;5(10):e13644. doi: 10.1371/journal.pone.0013644.
To secure pH homeostasis, the central respiratory network must permanently adapt its rhythmic motor drive to environment and behaviour. In neonates, it is commonly admitted that the retrotrapezoid/parafacial respiratory group of neurons of the ventral medulla plays the primary role in the respiratory response to acidosis, although the serotonergic system may also contribute to this response.
METHODOLOGY/PRINCIPAL FINDINGS: Using en bloc medullary preparations from neonatal mice, we have shown for the first time that the respiratory response to acidosis is abolished after pre-treatment with the serotonin-transporter blocker fluoxetine (25-50 µM, 20 min), a commonly used antidepressant. Using mRNA in situ hybridization and immunohistology, we have also shown the expression of the serotonin transporter mRNA and serotonin-containing neurons in the vicinity of the RTN/pFRG of neonatal mice.
These results reveal that the serotonergic system plays a pivotal role in pH homeostasis. Although obtained in vitro in neonatal mice, they suggest that drugs targeting the serotonergic system should be used with caution in infants, pregnant women and breastfeeding mothers.
为了确保 pH 值的体内平衡，中枢呼吸网络必须将其有节奏的运动驱动力永久地适应环境和行为。在新生儿中，人们普遍认为延髓腹外侧部的梯形/副基底呼吸神经元群在酸中毒时的呼吸反应中起着主要作用，尽管 5-羟色胺能系统也可能有助于这种反应。
方法/主要发现：使用来自新生小鼠的整体延髓标本，我们首次表明，用 5-羟色胺转运体阻滞剂氟西汀（25-50µM，20 分钟）预处理后，酸中毒引起的呼吸反应被消除。我们还使用 mRNA 原位杂交和免疫组织化学，显示了新生小鼠 RTN/pFRG 附近的 5-羟色胺转运体 mRNA 和含 5-羟色胺的神经元的表达。
这些结果表明，5-羟色胺能系统在 pH 值的体内平衡中起着关键作用。尽管是在新生小鼠的体外获得的，但它们表明，针对 5-羟色胺能系统的药物在婴儿、孕妇和哺乳期妇女中应谨慎使用。
