Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA.
Semin Thromb Hemost. 2010 Nov;36(8):845-56. doi: 10.1055/s-0030-1267038. Epub 2010 Nov 3.
All cell types shed ectosomes and exosomes, collectively known as microparticles (MP; 0.1 to 1.5 μm in diameter), when activated or stressed; normal human plasma contains ~2 μg MP protein/mL. The cellular composition of plasma MP is altered in many diseases, including acute coronary syndrome, diabetes mellitus, sepsis, and sickle cell disease. We measured the plasma MP protein composition of 42 patients (median age 69.5 years, most with cardiovascular disease) by label-free liquid chromatography coupled to tandem mass spectrometry. Among 458 proteins detected with high confidence (identified by at least two unique peptides with SEQUEST XCor (Thermo Electron Corp., San Jose, CA) ≥ 2.0, 2.2, and 3.3 for charge states +1, +2, and +3, respectively), 130 were present in most patients, representing a "core" set of plasma MP proteins. This core is enriched in cytoskeletal, integrin complex, and hemostasis proteins, and spectral counts of several proteins correlate with patient age and gender. We conclude that the MP proteome may be a useful and reliable source of biologically relevant disease biomarkers.
所有细胞类型在激活或应激时都会脱落胞外体和外泌体，统称为微颗粒（MP；直径 0.1 至 1.5 微米）；正常人体血浆中含有约 2 μg MP 蛋白/mL。血浆 MP 的细胞组成在许多疾病中发生改变，包括急性冠状动脉综合征、糖尿病、败血症和镰状细胞病。我们通过无标记液相色谱法与串联质谱联用，对 42 名患者（中位年龄 69.5 岁，大多数患有心血管疾病）的血浆 MP 蛋白组成进行了测量。在通过 SEQUEST XCor（Thermo Electron Corp.，San Jose，CA）值≥2.0、2.2 和 3.3（分别为+1、+2 和+3 的电荷状态）鉴定的 458 种高置信度检测到的蛋白质中，有 130 种存在于大多数患者中，代表了血浆 MP 蛋白质的“核心”集合。该核心富含细胞骨架、整合素复合物和止血蛋白，并且几种蛋白质的光谱计数与患者年龄和性别相关。我们得出结论，MP 蛋白质组可能是一种有用且可靠的生物相关疾病生物标志物来源。
