[体外培养条件对川贝母再生的影响]
[The effects of in vitro culture conditions on regeneration of Fritillaria cirrhosa].
作者信息
Wang Yue-Hua, Dai Yong, He Zong-Sheng, Sun Yan-Xia, Yan Sheng-Jie, Xu Shi-Jun, Wang Xiao-Rong
机构信息
Department of Biological Engineering, Chengdu University, Chengdu 610160, China.
出版信息
Zhong Yao Cai. 2010 Jun;33(6):854-6.
OBJECTIVE
The optimal in vitro culture conditions for the regeneration of Fritillaria cirrhosa were screened.
METHODS
Through the in vitro culture of the flowering stage Fritillaria cirrhosa, the effect of illumination, culture temperature, hormone combination and the amount of ribavirin on the regeneration of Fritillaria cirrhosa were studied.
RESULTS AND CONCLUSION
The explant browning level could be apparently reduced if the bulb were cultured under the ray after exposure to proper low-temperature. The optimal temperature for the regeneration of bulb is (20 +/- 2) degrees C. The hormone has obvious promotional effect on the regeneration of bulb, and the best combination is 6-BA 2.0 mg/L plus NAA 0.2 mg/L Concentration of 10 mg/L of ribavirin is best for the regeneration of bulb.
目的
筛选川贝母再生的最佳体外培养条件。
方法
通过对花期川贝母进行体外培养，研究光照、培养温度、激素组合及利巴韦林用量对川贝母再生的影响。
结果与结论
鳞茎经适当低温处理后在光照下培养，外植体褐化程度可明显降低。鳞茎再生的最适温度为（20±2）℃。激素对鳞茎再生有明显促进作用，最佳组合为6-BA 2.0 mg/L加NAA 0.2 mg/L。利巴韦林浓度为10 mg/L时最有利于鳞茎再生。
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