Leibniz Institute for Zoo and Wildlife Research (IZW), Research Group of Wildlife Diseases, Alfred-Kowalke-Str. 17, D-10315 Berlin, Germany.
Vet Microbiol. 2011 Apr 21;149(1-2):163-71. doi: 10.1016/j.vetmic.2010.10.002. Epub 2010 Nov 2.
Pasteurella are an important cause of fatal infections in free-ranging bats, but the genetic diversity of bat-derived strains is unclear. In the current study, 81 Pasteurella strains associated with pneumonia, severe organ necroses and systemic infection in free-ranging European vespertilionid bats were characterized by biochemical and molecular typing methods. Genetic relationships and subspecies status of Pasteurella multocida strains were determined by comparative 16S rDNA and rpoB gene sequence analysis. In addition, 30 representatives of the bat-derived P. multocida strains were selected based on phenotypic and genotypic tests to be compared by pulsed-field gel electrophoresis using SmaI. Most (85%) of the Pasteurella strains obtained from free-ranging bats in this study represented P. multocida ssp. septica. P. multocida ssp. multocida and Pasteurella species B were also identified in a small number of isolates. PFGE analysis correlated well with the sequencing results and revealed a high genetic diversity among bat-derived strains of P. multocida ssp. septica. Strains sharing identical or closely related SmaI fragment patterns were cultured from bats of different species, geographic origins, and years of isolation. The presence of numerous different P. multocida strains allows the assumption that Pasteurella infections in vespertilionid bats are not solely based on intra- but also on inter-species transmission. And indeed, our results present evidence of P. multocida infections in bats following cat predation.
巴斯德氏菌是自由栖息蝙蝠致命感染的重要原因，但蝙蝠来源的菌株遗传多样性尚不清楚。在目前的研究中，通过生化和分子分型方法对与自由栖息的欧洲蝙蝠科蝙蝠肺炎、严重器官坏死和全身性感染相关的 81 株巴斯德氏菌进行了特征描述。通过比较 16S rDNA 和 rpoB 基因序列分析，确定了多杀巴斯德氏菌菌株的遗传关系和亚种地位。此外，根据表型和基因型试验，从蝙蝠来源的 30 株多杀巴斯德氏菌菌株中选择代表菌株，并用 SmaI 进行脉冲场凝胶电泳比较。本研究中从自由栖息蝙蝠中获得的大多数（85%）巴斯德氏菌菌株代表杀多杀巴斯德氏菌亚种。在少数分离株中也鉴定出杀多杀巴斯德氏菌亚种和巴斯德氏菌 B。PFGE 分析与测序结果相关性良好，显示杀多杀巴斯德氏菌亚种的蝙蝠来源菌株具有很高的遗传多样性。具有相同或密切相关 SmaI 片段模式的菌株来自不同物种、地理来源和分离年份的蝙蝠。存在大量不同的多杀巴斯德氏菌菌株，这使得我们可以假设蝙蝠中的巴斯德氏菌感染不仅基于种内传播，还基于种间传播。事实上，我们的结果提供了猫捕食后蝙蝠感染多杀巴斯德氏菌的证据。
