Donnio P-Y, Allardet-Servent A, Perrin M, Escande F, Avril J-L
INSERM U341, Faculté de Médecine, 30000 Nimes.
Laboratoire des Pasteurella, Institut Pasteur, 75724 Paris, France.
J Med Microbiol. 1999 Feb;48(2):125-131. doi: 10.1099/00222615-48-2-125.
Thirty-six isolates, from man or swine, of Pasteurella multocida subsp. multocida producing (n = 13) or not producing (n = 23) the dermonecrotic toxin (DNT) were studied by numerical analysis, capsular typing and ribotyping. Toxigenic strains were also characterised by restriction fragment length polymorphism (RFLP) of the toxA gene and pulsed-field gel electrophoresis (PFGE). Numerical analysis differentiated the Pasteurella species and subspecies, but did not discriminate between toxigenic and nontoxigenic strains. RFLP demonstrated that toxA was located in a conserved part of the chromosome of all toxigenic strains. Ribotyping provided evidence of a close association between DNT production and one of the six EcoRI ribotypes designated as E2. In contrast, PFGE provided evidence for significant DNA polymorphism amongst the toxigenic strains. Results of phenotypic and genotypic studies suggested that toxigenic strains do not form a clone within the subspecies multocida. No difference was found between toxigenic strains of porcine or human origin by biochemical characterisation, capsular serotyping or genomic typing methods.
对36株来自人和猪的多杀巴斯德氏菌多杀亚种菌株进行了研究，其中13株产生皮肤坏死毒素（DNT），23株不产生该毒素，研究方法包括数值分析、荚膜分型和核糖分型。产毒菌株还通过toxA基因的限制性片段长度多态性（RFLP）和脉冲场凝胶电泳（PFGE）进行了特征分析。数值分析可区分巴斯德氏菌的种和亚种，但无法区分产毒菌株和不产毒菌株。RFLP表明toxA位于所有产毒菌株染色体的保守区域。核糖分型提供了证据，表明DNT产生与指定为E2的六种EcoRI核糖型之一密切相关。相比之下，PFGE显示产毒菌株之间存在显著的DNA多态性。表型和基因型研究结果表明，产毒菌株在多杀亚种内未形成一个克隆。通过生化特征分析、荚膜血清分型或基因组分型方法，未发现猪源或人源产毒菌株之间存在差异。
