Centro VISAVET, Universidad Complutense de Madrid, Madrid, Spain.
Vet J. 2011 Oct;190(1):131-5. doi: 10.1016/j.tvjl.2010.09.026. Epub 2010 Nov 2.
The interferon-gamma (IFN-γ) assay is an effective tool for the diagnosis of tuberculosis (Tb) in goats. The objectives of this study were to evaluate factors that might affect assay performance: (1) the phenol concentration of the purified protein derivative (PPD, tuberculin) used; (2) dialysis of PPD; and (3) delaying antigenic stimulation of blood samples for 8, 16 and 24h after collection. The assay was performed in duplicate with two cut-off points. Dialysis of PPD reduced test sensitivity, whereas the concentration of phenol did not significantly affect test outcome. Delaying antigenic stimulation of samples >8h resulted in a reduction in test sensitivity, compromising the capacity of the assay to detect infected animals. Performing the assay in duplicate was unnecessary, which has implications for reducing assay costs. These findings will facilitate the effective application of the IFN-γ assay as an ancillary test in Tb eradication programmes in goats.
干扰素-γ（IFN-γ）检测法是一种用于诊断山羊结核病（Tb）的有效工具。本研究旨在评估可能影响检测性能的因素：（1）使用的纯化蛋白衍生物（PPD，结核菌素）中的苯酚浓度；（2）PPD 的透析；以及（3）在采血后 8、16 和 24 小时延迟抗原刺激。该检测法采用双份样本和两个截断值进行。PPD 的透析会降低检测的敏感性，而苯酚的浓度对检测结果没有显著影响。样本的抗原刺激延迟超过 8 小时会导致检测敏感性降低，从而降低检测感染动物的能力。进行双份检测是不必要的，这对降低检测成本具有重要意义。这些发现将有助于 IFN-γ检测法作为山羊结核病根除计划中的辅助检测方法得到有效应用。
