Gutiérrez M, Tellechea J, García Marín J F
Dpto. Patología Animal, Medicina Animal, Facultad de Veterinaria, León, Spain.
Vet Microbiol. 1998 Aug 15;62(4):281-90. doi: 10.1016/s0378-1135(98)00217-x.
We have evaluated the comparative intradermal skin (CID) test, the interferon gamma (IFN-gamma) assay, two ELISAs with bovine PPD antigen, the standard and the anamnestic using sera obtained, respectively, at the time of the tuberculin injection and 15 days later, for the diagnosis of caprine tuberculosis (TB). The sensitivity and specificity results were high for the CID test (83.7%, 100%), the IFN-gamma assay (83.7%, 96%) and the anamnestic ELISA (88.6%, 95.8%). In contrast, they were comparatively low for the standard ELISA (54.9%, 88%). However, test results with the standard ELISA were positive in a group of goats with cavitating TB (100%). A combination of the CID test and the IFN-gamma assay offered the highest sensitivity, 95.8%, and also high specificity, 96%. In spite of this, the evidence that the serological tests were most sensitive for the detection of goats with severe lesions (100% positivity) suggested that a combination of CID test and anamnestic ELISA may be most useful as part of an eradication campaign against caprine TB.
我们评估了比较皮内皮肤（CID）试验、干扰素γ（IFN-γ）检测、两种使用牛结核菌素纯蛋白衍生物（PPD）抗原的酶联免疫吸附测定（ELISA），即标准ELISA和回忆性ELISA，分别使用在结核菌素注射时和15天后采集的血清来诊断山羊结核病（TB）。CID试验（83.7%，100%）、IFN-γ检测（83.7%，96%）和回忆性ELISA（88.6%，95.8%）的敏感性和特异性结果较高。相比之下，标准ELISA的敏感性和特异性结果相对较低（54.9%，88%）。然而，标准ELISA在一组有空洞性结核病的山羊中的检测结果呈阳性（100%）。CID试验和IFN-γ检测相结合具有最高的敏感性，为95.8%，特异性也较高，为96%。尽管如此，血清学检测对检测有严重病变的山羊最为敏感（阳性率100%）这一证据表明，CID试验和回忆性ELISA相结合作为山羊结核病根除运动的一部分可能最为有用。
