Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Campus Regional de Excelencia Internacional, Campus Mare Nostrum, Universidad de Murcia, Spain.
Res Vet Sci. 2013 Dec;95(3):1107-13. doi: 10.1016/j.rvsc.2013.10.003. Epub 2013 Oct 16.
Caprine tuberculosis is a major health problem for goats, and an important zoonosis. Eradication programmes using the comparative skin test are being developed to aid in decreasing the prevalence of infection. However, persistent tuberculosis infections are found in herds subjected to eradication programmes. In the present study a commercial IFN-γ release assay and an experimental ELISA based on MPB70, were evaluated as potential ancillary tests to detect infection. The relationship between the three techniques (skin test, IFN-γ release and ELISA) and histopathological lesions was analyzed in 162 goats from herds with persistent tuberculosis infection. The presence of related pathogens (paratuberculosis and pseudotuberculosis) was also studied. The IFN-γ release assay and the ELISA had a higher sensitivity than the comparative skin test (65.3% and 66.3% vs 44.5%) using as a gold standard a combination of histopathological analysis and isolation. Used in combination, ELISA and the skin test detected 89.1% of goats with tuberculosis while a combination of IFN-γ assay and skin test detected 78.2%. The types of macroscopic and microscopic lesions reflected the results of diagnostic tests.
山羊结核病是山羊的主要健康问题，也是一种重要的人畜共患病。正在制定使用比较皮肤试验的根除计划，以帮助降低感染的流行率。然而，在接受根除计划的畜群中仍发现持续的结核病感染。在本研究中，评估了一种商业 IFN-γ 释放测定法和一种基于 MPB70 的实验 ELISA，作为检测感染的潜在辅助检测方法。在来自持续结核病感染畜群的 162 只山羊中，分析了三种技术（皮肤试验、IFN-γ 释放和 ELISA）与组织病理学病变之间的关系。还研究了相关病原体（副结核病和假结核病）的存在。使用组织病理学分析和分离的组合作为金标准，IFN-γ 释放测定法和 ELISA 的敏感性高于比较皮肤试验（65.3%和 66.3%对 44.5%）。联合使用 ELISA 和皮肤试验检测到 89.1%的结核病山羊，而 IFN-γ 测定法和皮肤试验联合检测到 78.2%的结核病山羊。宏观和微观病变的类型反映了诊断测试的结果。
