Department of Nursing, University of Granada, Spain.
Acta Pharmacol Sin. 2010 Nov;31(11):1495-9. doi: 10.1038/aps.2010.129.
To examine the effects of acetaminophen (paracetamol), a nonsteroidal anti-inflammatory drug (NSAID), on different cellular and functional parameters of the human osteosarcoma cell line MG63.
Flow cytometry was used to study proliferation, antigenic profile, and phagocytic activity, and radioimmunoassay was used to determine osteocalcin synthesis as a cell differentiation marker.
Short-term treatment with therapeutic doses of paracetamol(5 or 25 μmol/L) reduced cell proliferation, osteocalcin synthesis, and phagocyte activity, and increased the expression of antigens involved in antigen presentation to T lymphocytes (CD80, CD86, HLA-DR).
These findings suggest that paracetamol activates the osteoblast, inducing its immunogenic action to the detriment of its bone formation capacity.
研究对乙酰氨基酚(扑热息痛)这一非甾体类抗炎药(NSAID)对人骨肉瘤细胞系 MG63 的不同细胞和功能参数的影响。
采用流式细胞术研究增殖、抗原表型和吞噬活性,放射免疫法测定作为细胞分化标志物的骨钙素合成。
短期治疗剂量的对乙酰氨基酚(5 或 25 μmol/L)降低细胞增殖、骨钙素合成和吞噬活性,并增加与向 T 淋巴细胞呈递抗原相关的抗原表达(CD80、CD86、HLA-DR)。
这些发现表明,对乙酰氨基酚激活成骨细胞,诱导其免疫原性作用,损害其成骨能力。