• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

阿司匹林对人MG-63骨肉瘤细胞系细胞生长的影响。

Effect of aspirin on cell growth of human MG-63 osteosarcoma line.

作者信息

De Luna-Bertos E, Ramos-Torrecillas J, García-Martínez O, Díaz-Rodríguez L, Ruiz C

机构信息

Biomedical Group, BIO277, Department of Nursing, Faculty of Health Sciences, University of Granada, 18071 Granada, Spain.

出版信息

ScientificWorldJournal. 2012;2012:834246. doi: 10.1100/2012/834246. Epub 2012 May 2.

DOI:10.1100/2012/834246
PMID:22654634
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3354653/
Abstract

Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used in bone tissue repair treatment for their pharmacological action. The objective of this study was to determine the effect of aspirin, on osteoblast growth, using MG63 cell line as osteoblast model. MTT spectrophotometry results showed that 20, 100, and 1000 μM aspirin doses have an inhibitory effect on growth. Cell cycle analysis revealed that aspirin doses of 100 and 1000 μM arrest the cell cycle in phase GO/G1. Parallel apoptosis/necrosis studies showed no changes in comparison to control cells after treatment with 1 or 10 μM aspirin but a significantly increased percentage of cells in apoptosis at doses of 20, 100, and 1000 μM. We highlight that treatment of osteoblast-like cells with 1000 μM aspirin increased not only the percentage of cells in apoptosis but also the percentage of necrotic cells, which was not observed in aspirin treatments at lower doses.

摘要

非甾体抗炎药（NSAIDs）因其药理作用而常用于骨组织修复治疗。本研究的目的是使用MG63细胞系作为成骨细胞模型，确定阿司匹林对成骨细胞生长的影响。MTT分光光度法结果显示，20、100和1000μM的阿司匹林剂量对生长有抑制作用。细胞周期分析表明，100和1000μM的阿司匹林剂量使细胞周期停滞在G0/G1期。平行的凋亡/坏死研究表明，与对照细胞相比，用1或10μM阿司匹林处理后没有变化，但在20、100和1000μM剂量下，凋亡细胞的百分比显著增加。我们强调，用1000μM阿司匹林处理成骨样细胞不仅增加了凋亡细胞的百分比，还增加了坏死细胞的百分比，而在较低剂量的阿司匹林处理中未观察到这种情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8389/3354653/ffe147c1a7cc/TSWJ2012-834246.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8389/3354653/39bb4e74c39c/TSWJ2012-834246.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8389/3354653/780b7c51c35c/TSWJ2012-834246.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8389/3354653/ffe147c1a7cc/TSWJ2012-834246.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8389/3354653/39bb4e74c39c/TSWJ2012-834246.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8389/3354653/780b7c51c35c/TSWJ2012-834246.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8389/3354653/ffe147c1a7cc/TSWJ2012-834246.003.jpg

相似文献

1
Effect of aspirin on cell growth of human MG-63 osteosarcoma line.阿司匹林对人MG-63骨肉瘤细胞系细胞生长的影响。
ScientificWorldJournal. 2012;2012:834246. doi: 10.1100/2012/834246. Epub 2012 May 2.
2
Effects on growth of human osteoblast-like cells of three nonsteroidal anti-inflammatory drugs: metamizole, dexketoprofen, and ketorolac.三种非甾体抗炎药：安乃近、右酮洛芬和酮咯酸对人成骨样细胞生长的影响。
Biol Res Nurs. 2015 Jan;17(1):62-7. doi: 10.1177/1099800414527155. Epub 2014 Mar 12.
3
High doses of bisphosphonates reduce osteoblast-like cell proliferation by arresting the cell cycle and inducing apoptosis.高剂量双膦酸盐通过使细胞周期停滞并诱导细胞凋亡来降低成骨样细胞的增殖。
J Craniomaxillofac Surg. 2015 Apr;43(3):396-401. doi: 10.1016/j.jcms.2014.12.008. Epub 2014 Dec 19.
4
[Effect of aspirin on cell biological activities in murine bone marrow stromal cells].[阿司匹林对小鼠骨髓基质细胞生物学活性的影响]
Zhonghua Kou Qiang Yi Xue Za Zhi. 2016 Mar;51(3):160-5. doi: 10.3760/cma.j.issn.1002-0098.2016.03.007.
5
Gambogic acid inhibits the growth of osteosarcoma cells in vitro by inducing apoptosis and cell cycle arrest.藤黄酸通过诱导细胞凋亡和细胞周期阻滞抑制骨肉瘤细胞的体外生长。
Oncol Rep. 2011 May;25(5):1289-95. doi: 10.3892/or.2011.1189. Epub 2011 Feb 17.
6
Metformin synergistically enhances antitumor activity of histone deacetylase inhibitor trichostatin a against osteosarcoma cell line.二甲双胍与组蛋白去乙酰化酶抑制剂曲古抑菌素 A 协同增强对骨肉瘤细胞系的抗肿瘤活性。
DNA Cell Biol. 2013 Apr;32(4):156-64. doi: 10.1089/dna.2012.1926. Epub 2013 Mar 1.
7
Overexpression of miR-126 sensitizes osteosarcoma cells to apoptosis induced by epigallocatechin-3-gallate.miR-126的过表达使骨肉瘤细胞对表没食子儿茶素-3-没食子酸酯诱导的凋亡敏感。
World J Surg Oncol. 2014 Dec 16;12:383. doi: 10.1186/1477-7819-12-383.
8
Nitric Oxide-Releasing Aspirin Suppresses NF-κB Signaling in Estrogen Receptor Negative Breast Cancer Cells in Vitro and in Vivo.释放一氧化氮的阿司匹林在体外和体内均可抑制雌激素受体阴性乳腺癌细胞中的NF-κB信号通路。
Molecules. 2015 Jul 9;20(7):12481-99. doi: 10.3390/molecules200712481.
9
Mechanisms underlying aspirin-mediated growth inhibition and apoptosis induction of cyclooxygenase-2 negative colon cancer cell line SW480.阿司匹林介导的环氧化酶-2阴性结肠癌细胞系SW480生长抑制和凋亡诱导的潜在机制。
World J Gastroenterol. 2008 Jul 14;14(26):4227-33. doi: 10.3748/wjg.14.4227.
10
Nonsteroidal antiinflammatory drugs inhibit the proliferation of colon adenocarcinoma cells: effects on cell cycle and apoptosis.非甾体抗炎药抑制结肠腺癌细胞的增殖：对细胞周期和细胞凋亡的影响。
Exp Cell Res. 1996 Jan 10;222(1):179-88. doi: 10.1006/excr.1996.0023.

引用本文的文献

1
Aspirin in Cancer Therapy: Pharmacology and Nanotechnology Advances.阿司匹林在癌症治疗中的应用：药理学与纳米技术进展
Int J Nanomedicine. 2025 Feb 23;20:2327-2365. doi: 10.2147/IJN.S505636. eCollection 2025.
2
Does chronic low-dose aspirin use benefit bone health? A cross-sectional study on patients with type 2 diabetes mellitus.慢性低剂量阿司匹林的使用是否有益于骨骼健康？一项关于 2 型糖尿病患者的横断面研究。
BMC Endocr Disord. 2023 Apr 7;23(1):79. doi: 10.1186/s12902-023-01309-2.
3
Gene expression profiling on effect of aspirin on osteogenic differentiation of periodontal ligament stem cells.

本文引用的文献

1
Effects of paracetamol, non-steroidal anti-inflammatory drugs, acetylsalicylic acid, and opioids on bone mineral density and risk of fracture: results of the Danish Osteoporosis Prevention Study (DOPS).对乙酰氨基酚、非甾体抗炎药、乙酰水杨酸和阿片类药物对骨密度和骨折风险的影响：丹麦骨质疏松预防研究（DOPS）的结果。
Osteoporos Int. 2012 Apr;23(4):1255-65. doi: 10.1007/s00198-011-1692-0. Epub 2011 Jun 28.
2
Effects of indomethacin, nimesulide, and diclofenac on human MG-63 osteosarcoma cell line.吲哚美辛、尼美舒利和双氯芬酸对人 MG-63 骨肉瘤细胞系的影响。
Biol Res Nurs. 2012 Jan;14(1):98-107. doi: 10.1177/1099800411398933. Epub 2011 Mar 8.
3
基因表达谱分析阿司匹林对牙周膜干细胞成骨分化的影响
BDJ Open. 2021 Sep 16;7(1):35. doi: 10.1038/s41405-021-00090-5.
4
Dose-dependent roles of aspirin and other non-steroidal anti-inflammatory drugs in abnormal bone remodeling and skeletal regeneration.阿司匹林及其他非甾体抗炎药在异常骨重塑和骨骼再生中的剂量依赖性作用。
Cell Biosci. 2019 Dec 23;9:103. doi: 10.1186/s13578-019-0369-9. eCollection 2019.
5
Lower dosage of aspirin promotes cell growth and osteogenic differentiation in murine bone marrow stromal cells.低剂量阿司匹林可促进小鼠骨髓基质细胞的生长和成骨分化。
J Dent Sci. 2016 Sep;11(3):315-322. doi: 10.1016/j.jds.2016.03.009. Epub 2016 May 13.
6
Aspirin promotes apoptosis and inhibits proliferation by blocking G0/G1 into S phase in rheumatoid arthritis fibroblast-like synoviocytes via downregulation of JAK/STAT3 and NF-κB signaling pathway.阿司匹林通过下调 JAK/STAT3 和 NF-κB 信号通路，阻止 G0/G1 期进入 S 期，从而促进类风湿关节炎成纤维样滑膜细胞凋亡并抑制增殖。
Int J Mol Med. 2018 Dec;42(6):3135-3148. doi: 10.3892/ijmm.2018.3883. Epub 2018 Sep 17.
7
Aspirin is Involved in the Cell Cycle Arrest, Apoptosis, Cell Migration, and Invasion of Oral Squamous Cell Carcinoma.阿司匹林参与口腔鳞状细胞癌的细胞周期停滞、细胞凋亡、细胞迁移和侵袭。
Int J Mol Sci. 2018 Jul 12;19(7):2029. doi: 10.3390/ijms19072029.
8
Advances in limb salvage treatment of osteosarcoma.骨肉瘤保肢治疗的进展
J Bone Oncol. 2017 Nov 26;10:36-40. doi: 10.1016/j.jbo.2017.11.005. eCollection 2018 Mar.
9
Phenolic Compounds in Extra Virgin Olive Oil Stimulate Human Osteoblastic Cell Proliferation.特级初榨橄榄油中的酚类化合物刺激人成骨细胞增殖。
PLoS One. 2016 Mar 1;11(3):e0150045. doi: 10.1371/journal.pone.0150045. eCollection 2016.
10
Synthesis and biological activity of NOSH-naproxen (AVT-219) and NOSH-sulindac (AVT-18A) as potent anti-inflammatory agents with chemotherapeutic potential.具有化疗潜力的强效抗炎药NOSH-萘普生（AVT-219）和NOSH-舒林酸（AVT-18A）的合成及生物活性
Medchemcomm. 2013;4(11). doi: 10.1039/C3MD00185G.
Effect of acetaminophen, ibuprofen and methylprednisolone on different parameters of human osteoblast-like cells.
对乙酰氨基酚、布洛芬和甲泼尼龙对人成骨样细胞不同参数的影响。
Arch Oral Biol. 2011 Apr;56(4):317-23. doi: 10.1016/j.archoralbio.2010.10.018. Epub 2010 Nov 26.
4
Effects of non-steroidal anti-inflammatory drugs on proliferation, differentiation and migration in equine mesenchymal stem cells.非甾体抗炎药对马间充质干细胞增殖、分化和迁移的影响。
Cell Biol Int. 2011 Mar;35(3):235-48. doi: 10.1042/CBI20090211.
5
Effect of acetaminophen (paracetamol) on human osteosarcoma cell line MG63.对乙酰氨基酚（扑热息痛）对人骨肉瘤细胞株 MG63 的影响。
Acta Pharmacol Sin. 2010 Nov;31(11):1495-9. doi: 10.1038/aps.2010.129.
6
Mechanisms behind COX-1 and COX-2 inhibition of tumor growth in vivo.COX-1 和 COX-2 抑制体内肿瘤生长的机制。
Int J Oncol. 2010 Nov;37(5):1143-52. doi: 10.3892/ijo_00000766.
7
Immunomodulatory effects of diclofenac in leukocytes through the targeting of Kv1.3 voltage-dependent potassium channels.通过靶向 Kv1.3 电压依赖性钾通道，双氯芬酸对白细胞的免疫调节作用。
Biochem Pharmacol. 2010 Sep 15;80(6):858-66. doi: 10.1016/j.bcp.2010.05.012. Epub 2010 May 19.
8
Effect of ibuprofen on osteoblast differentiation of porcine bone marrow-derived progenitor cells.布洛芬对猪骨髓源祖细胞成骨细胞分化的影响。
J Oral Maxillofac Surg. 2009 Nov;67(11):2412-7. doi: 10.1016/j.joms.2009.05.434.
9
Antigenic phenotype and phagocytic capacity of MG-63 osteosarcoma line.MG-63骨肉瘤细胞系的抗原表型和吞噬能力
Ann N Y Acad Sci. 2009 Sep;1173 Suppl 1:E46-54. doi: 10.1111/j.1749-6632.2009.04950.x.
10
Controversial effects of non-steroidal anti-inflammatory drugs on bone: a review.非甾体抗炎药对骨骼的争议性影响：综述
Inflamm Allergy Drug Targets. 2009 Jul;8(3):169-75. doi: 10.2174/187152809788681065.