Garner L L, Campbell G T, Blake C A
Department of Anatomy, Cell Biology and Neurosciences, University of South Carolina School of Medicine, Columbia 29208.
Endocrinology. 1990 Feb;126(2):992-100. doi: 10.1210/endo-126-2-992.
We investigated whether chronic administration of LHRH to normal adult rats could increase the percentages of anterior pituitary gland (APG) cells that contain immunoreactive LH and/or FSH and gonadotropin secretion. Vehicle or 1 microgram LHRH was injected sc twice daily for 6 days, and rats were decapitated 16 h after the last injection. Treatment with LHRH caused nearly a doubling in the numerical density of LH and FSH cells and in the percentage of APG cells that contained LH or FSH. It also caused a shift in the gonadotroph population from LH and LH/FSH cells to LH/FSH cells. It did not change the mean size of gonadotrophs or APG weight. These changes at the light microscopic level were not accompanied by any apparent changes in LH cells at the ultrastructural level. However, they were accompanied by an approximate doubling of the basal serum LH and FSH concentrations, an increase in the APG FSH concentration, and an increase in the basal FSH release rate (measured in vitro). The results indicate that exogenous LHRH can be administered to increase numbers of gonadotrophs in the APG, synthesis of FSH in gonadotrophs, and basal serum LH and FSH concentrations.
我们研究了对正常成年大鼠长期给予促性腺激素释放激素（LHRH）是否会增加垂体前叶（APG）中含有免疫反应性促黄体生成素（LH）和/或促卵泡生成素（FSH）的细胞百分比以及促性腺激素的分泌。每天两次皮下注射赋形剂或1微克LHRH，持续6天，在最后一次注射后16小时将大鼠断头。LHRH处理导致LH和FSH细胞的数量密度以及含有LH或FSH的APG细胞百分比几乎增加一倍。它还导致促性腺激素细胞群体从LH和LH/FSH细胞转变为LH/FSH细胞。它没有改变促性腺激素细胞的平均大小或APG重量。光镜水平的这些变化在超微结构水平上并未伴随LH细胞出现任何明显变化。然而，它们伴随着基础血清LH和FSH浓度大约增加一倍、APG中FSH浓度增加以及基础FSH释放率增加（体外测量）。结果表明，给予外源性LHRH可以增加APG中促性腺激素细胞的数量、促性腺激素细胞中FSH的合成以及基础血清LH和FSH浓度。
