Lindgren V, Ostroff R M, Vasil M L, Wretlind B
Department of Clinical Bacteriology, Danderyd Hospital, Sweden.
J Bacteriol. 1990 Feb;172(2):1155-6. doi: 10.1128/jb.172.2.1155-1156.1990.
An insertion mutation constructed by gene replacement methods was used to map the gene corresponding to the hemolytic phospholipase C (plcS gene) in Pseudomonas aeruginosa PAO1 by R68.45-mediated conjugation. plcS mapped approximately at 67 min on the 75-min chromosomal map (B. W. Holloway, K. O'Hoy, and H. Matsumoto, p. 213-221, in S. J. O'Brien, ed., Genetic Maps 1987, vol. 4, 1987), between the markers pur-67 and pru-375 and considerably distal to the regulatory genes plcA and plcB, which are located at approximately 12 min.
通过基因置换方法构建的插入突变用于通过R68.45介导的接合来定位铜绿假单胞菌PAO1中与溶血磷脂酶C相对应的基因（plcS基因）。plcS大约定位在75分钟染色体图谱的67分钟处（B. W. 霍洛韦、K. 奥霍伊和H. 松本，第213 - 221页，载于S. J. 奥布赖恩编，《遗传图谱1987》，第4卷，1987年），位于标记pur - 67和pru - 375之间，且远于位于大约12分钟处的调控基因plcA和plcB。
