利用Rfla质粒和接合作用分析铜绿假单胞菌中的鞭毛基因。
Analysis of flagellar genes in Pseudomonas aeruginosa by use of Rfla plasmids and conjugations.
作者信息
Tsuda M, Oguchi T, Iino T
出版信息
J Bacteriol. 1981 Sep;147(3):1008-14. doi: 10.1128/jb.147.3.1008-1014.1981.
Abstract
Over 300 flagellar mutants were isolated in Pseudomonas aeruginosa PAO. R-prime plasmids carrying segments of bacterial chromosome which can complement the mutant phenotypes were isolated by means of plasmid R68.45. Among the R-prime plasmids, pMT6 complemented 167 out of 307 mutants examined, and pMT19 complemented the remaining 140 mutants. We found no mutant which was complemented by both of these plasmids. Hence, the flagellar genes were divided into two clusters by these two plasmids, namely, region I on pMT19 and region II on pMT6. By FP5- and R68.45-mediated conjugation, these two regions were located on the P. aeruginosa PAO chromosome with an order of puuF--region I--region II--oru-325.
摘要
在铜绿假单胞菌PAO中分离出了300多个鞭毛突变体。通过质粒R68.45分离出携带可互补突变体表型的细菌染色体片段的R-prime质粒。在这些R-prime质粒中，pMT6对307个检测突变体中的167个进行了互补，pMT19对其余140个突变体进行了互补。我们没有发现被这两种质粒同时互补的突变体。因此，鞭毛基因被这两种质粒分为两个簇，即pMT19上的区域I和pMT6上的区域II。通过FP5和R68.45介导的接合作用，这两个区域位于铜绿假单胞菌PAO染色体上，顺序为puuF--区域I--区域II--oru-325。
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