两种基因交换方法在确定铜绿假单胞菌弹性蛋白酶结构基因遗传位点中的比较。
Comparison of two methods of genetic exchange in determination of the genetic locus of the structural gene for Pseudomonas aeruginosa elastase.
作者信息
Howe T R, Wretlind B, Iglewski B H
出版信息
J Bacteriol. 1983 Oct;156(1):58-61. doi: 10.1128/jb.156.1.58-61.1983.
Abstract
A mutation, lasA1, that results in the synthesis of enzymatically inactive elastase protein was mapped by R68.45 plasmid-mediated conjugation and transposon-facilitated recombination to ca. 75 min on the Pseudomonas aeruginosa PAO chromosome. Since immunologically cross-reactive material is produced, the mutation presumably arose within the elastase structural gene.
摘要
一种导致合成无酶活性弹性蛋白酶蛋白的突变体lasA1，通过R68.45质粒介导的接合和转座子促进的重组定位到铜绿假单胞菌PAO染色体上约75分钟处。由于产生了免疫交叉反应物质，推测该突变发生在弹性蛋白酶结构基因内。
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Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase.产弹性蛋白酶改变的铜绿假单胞菌PAO突变体的分离与鉴定
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