Ge Ruifeng, Ahn Jin-Chul, Shin Jang-In, Bahk Chan Woong, He Peijie, Chung Phil-Sang
Medical Laser Research Center, Dankook University, Cheonan, Chungnam, Korea.
Photomed Laser Surg. 2011 Mar;29(3):155-60. doi: 10.1089/pho.2009.2750. Epub 2010 Nov 6.
This study was designed to evaluate the anticancer effect of cisplatin and photodynamic therapy (PDT) combined in vitro and in vivo.
PDT, these days, is a promising modality for the treatment of cancer and infections. In order to optimize the treatment, cisplatin is often combined with other chemotherapeutic agents.
Colon cancer cells were incubated with cisplatin (0.1, 1, and 6 μg/ml), followed by photosensitization with Photogem® and irradiation with a 632 nm diode laser at an energy density of 3.2 J/cm(2). An MTT assay was then used to measure cell viability. For in vivo studies, established tumors were treated with cisplatin (3 mg/kg) alone or with PDT (5 mg/kg of Photogem®, 600 J/cm(2)). The sizes of the tumors were continuously measured to note the effects.
The cell viability of the combined therapy group was 19.88 ± 0.41, corresponding to a 9% increase compared with that of the cisplatin- or PDT-only groups. In vivo, the tumors treated with PDT or combination therapy disappeared completely three days after each treatment, but on the 14th day, the recurrence rate was significantly lower in the combination therapy group than in the PDT group.
Combination therapy results in an enhanced anticancer effect, presenting the possibility of minimizing the administration dosage of Photogem® and cisplatin.
本研究旨在评估顺铂与光动力疗法（PDT）联合应用在体外和体内的抗癌效果。
如今，光动力疗法是一种治疗癌症和感染的有前景的方法。为了优化治疗，顺铂常与其他化疗药物联合使用。
将结肠癌细胞与顺铂（0.1、1和6μg/ml）孵育，随后用Photogem®进行光敏化处理，并用能量密度为3.2J/cm²的632nm二极管激光照射。然后使用MTT法测量细胞活力。对于体内研究，已形成的肿瘤分别用单独的顺铂（3mg/kg）或光动力疗法（5mg/kg的Photogem®，600J/cm²）进行治疗。持续测量肿瘤大小以观察效果。
联合治疗组的细胞活力为19.88±0.41，与仅使用顺铂或光动力疗法的组相比，增加了9%。在体内，接受光动力疗法或联合治疗的肿瘤在每次治疗后三天完全消失，但在第14天，联合治疗组的复发率明显低于光动力疗法组。
联合治疗可增强抗癌效果，这表明有可能将Photogem®和顺铂的给药剂量降至最低。
