Morphology Department, School of Biochemical and Pharmaceutical Sciences, National University of Rosario, Suipacha 531, 2000 Rosario, Santa Fe, Argentina.
Neurotoxicol Teratol. 2011 Mar-Apr;33(2):297-302. doi: 10.1016/j.ntt.2010.10.010. Epub 2010 Nov 3.
In the present work, in vivo ROS formation and the activity of antioxidant enzymes in the hippocampus and the cerebellum of sodium metavanadate (NaVO₃) treated rats were studied. Rats were i.p. injected with 3 mg/kg bw/day (V₁ group) or with 7.2 mg/kg bw/day of NaVO₃ (V₂ group) for 5 consecutive days. Results show that after only 5 days of NaVO₃ exposure, reactive oxygen species formation and alteration of the oxidative defence system were observed. Vanadium-induced OH production was detected in cerebellum at the high dose. This result was confirmed by in situ ROS histochemical staining. Neither Cat nor Cu-Zn SOD activities showed changes while GSH/GSSG ratio, in both brain areas, was significantly decreased in NaVO₃-treated groups. The present work indicates that the NaVO₃ dose and the particular brain area constitution would be critical in the cellular and molecular oxidative mechanism of this element.
在本研究中，我们研究了五氧化二钒（NaVO₃）处理大鼠海马和小脑组织中的活性氧（ROS）形成和抗氧化酶活性。大鼠连续 5 天每天腹腔注射 3 毫克/千克体重（V₁ 组）或 7.2 毫克/千克体重（V₂ 组）的 NaVO₃。结果表明，仅在暴露于 NaVO₃ 5 天后，就观察到活性氧的形成和氧化防御系统的改变。在高剂量下，小脑组织中检测到钒诱导的 OH 生成。原位 ROS 组织化学染色证实了这一结果。两种 SOD（Cu-Zn SOD 和 Cat）的活性没有变化，而 GSH/GSSG 比值在两个脑区均显著降低。本研究表明，NaVO₃的剂量和特定脑区组成对于该元素的细胞和分子氧化机制至关重要。
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