Wnuk Ewa, Zwolak Iwona
Department of Biomedicine and Environmental Research, The John Paul II Catholic University of Lublin, Konstantynów Ave. 1J, 20-708 Lublin, Poland.
Molecules. 2025 May 9;30(10):2114. doi: 10.3390/molecules30102114.
Environmental pollution by heavy metals (HMs) has become a serious threat in recent years due to their potential consequences for human health and life. One such metal is vanadium (V). Despite its numerous benefits-including antibacterial, antifungal, and anticancer properties-V induces cellular damage through oxidative stress. Epigallocatechin gallate (EGCG), a potent antioxidant found in large quantities in green tea, is considered an effective protector against the damaging effects of HMs on cells. The aim of this study was to evaluate the possible effect of EGCG on CHO-K1 cells exposed to V. This is the first experiment of its kind on healthy cells. Cells were treated with V and EGCG for 24 h, either in combination or separately. The doses were selected in a preliminary stage of the experiment (V 50 and 100 µM; EGCG 0.5 and 1 µM). As part of the study, the cell viability, total ROS activity, and mitochondrial membrane potential were assessed. The results showed that at the tested concentrations, EGCG did not reduce the toxic effect of V on cells, but in fact exacerbated its adverse effects on cells. Further studies are needed to understand the exact mechanism of V-EGCG interaction in mammalian cells.
近年来，重金属对环境的污染因其对人类健康和生命的潜在影响而成为一个严重威胁。钒（V）就是这样一种金属。尽管钒有诸多益处，包括抗菌、抗真菌和抗癌特性，但它会通过氧化应激诱导细胞损伤。表没食子儿茶素没食子酸酯（EGCG）是绿茶中大量存在的一种强效抗氧化剂，被认为是对抗重金属对细胞损伤作用的有效保护剂。本研究的目的是评估EGCG对暴露于钒的CHO-K1细胞可能产生的影响。这是首次针对健康细胞进行此类实验。细胞分别或联合用钒和EGCG处理24小时。实验的初步阶段选定了剂量（钒50和100微摩尔；EGCG 0.5和1微摩尔）。作为研究的一部分，评估了细胞活力、总活性氧水平和线粒体膜电位。结果表明，在所测试的浓度下，EGCG并未降低钒对细胞的毒性作用，实际上反而加剧了其对细胞的不利影响。需要进一步研究以了解钒与EGCG在哺乳动物细胞中的相互作用的确切机制。
