Burdock Group, Orlando, FL 32801, USA.
Food Chem Toxicol. 2011 Jan;49(1):251-8. doi: 10.1016/j.fct.2010.10.027. Epub 2010 Nov 5.
Lactobacillus pentosus has a long history of use in cooked and uncooked fermented foods. Viable and heat-killed nonviable preparations of L. pentosus strain b240 were evaluated for short term and subchronic toxicity and genotoxic potential. Dose levels were determined through acute oral toxicity tests with viable (LD(50)>2500 mg/kg) and nonviable (LD(50)>2000 mg/kg) b240. In the short term study, rats received 2500 mg/kg/day (∼1.7×10(11)cfu/kg/day) viable b240 for 28 days. In the subchronic study, rats received 500, 1000 or 2000 mg/kg/day (up to ∼3.0×10(12) cfu equivalents/kg/day) nonviable b240 for 91 days followed by a 28-day recovery. No mortalities occurred. No treatment-related effects were identified for general condition, body weight, food-water consumption, ophthalmology, urinalysis, hematology, blood chemistry, organ weights, histopathology and gross pathology. Although statistically significant effects were noted for several endpoints in the short term and subchronic studies, none were related to the test materials. The NOAEL for nonviable b240 was 2000 mg/kg/day, the highest dose tested. Additionally, nonviable b240 (≤ 5000 μg/plate) was not mutagenic in Salmonella typhimurium or Escherichia coli tester strains nor did nonviable b240 orally administered to rats at levels ≤ 2000 mg/kg/day for two days, induce a clastogenic response.
戊糖片球菌在熟食和未熟发酵食品中有着悠久的应用历史。对戊糖片球菌 b240 的活菌和热灭活非活菌制剂进行了短期和亚慢性毒性和遗传毒性研究。通过活菌（LD50>2500mg/kg）和非活菌（LD50>2000mg/kg）b240 的急性口服毒性试验确定了剂量水平。在短期研究中，大鼠接受 2500mg/kg/天（约 1.7×10（11）cfu/kg/天）活菌 b240，为期 28 天。在亚慢性研究中，大鼠接受 500、1000 或 2000mg/kg/天（最高达约 3.0×10（12）cfu 当量/kg/天）非活菌 b240，为期 91 天，随后进行 28 天恢复期。没有死亡。一般状况、体重、食物-水消耗、眼科、尿液分析、血液学、血液化学、器官重量、组织病理学和大体病理学均未发现与治疗相关的影响。尽管在短期和亚慢性研究中，几个终点都出现了统计学上的显著影响，但都与试验材料无关。非活菌 b240 的无观察到不良作用水平为 2000mg/kg/天，为最高测试剂量。此外，非活菌 b240（≤5000μg/平板）在鼠伤寒沙门氏菌和大肠杆菌测试菌株中没有致突变性，也没有在大鼠中口服给予非活菌 b240 （≤2000mg/kg/天）两天，诱导断裂剂反应。
