Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
Food Microbiol. 2011 Feb;28(1):137-40. doi: 10.1016/j.fm.2010.09.006. Epub 2010 Sep 16.
Vibrio parahaemolyticus is a potentially pathogenic bacterium that occurs naturally in estuarine environments worldwide, and is often associated with gastroenteritis in humans following consumption of raw bivalve mollusks, especially raw oysters. The occurrence of total and pathogenic V. parahaemolyticus in 74 samples of raw oysters collected in restaurants, supermarkets, groceries and beach huts in Sao Paulo State, was monitored between February 2006 and January 2007. Enumeration of V. parahaemolyticus was performed according to the most probable number (MPN) procedure. Five to ten typical colonies were selected from thiosulfate-citrate-bile salts-sucrose (TCBS) agar plates for confirmation by the presence of the species-specific gene tlh and the virulence genes tdh and trh by multiplex PCR. V. parahaemolyticus was detected in 100% of samples. The densities of total V. parahaemolyticus varied from 1.78 to 6.04 log(10) (MPN/g), with higher densities being detected in fall and summer, and lower densities in winter (P < 0.05). There was no statistical difference among densities of V parahaemolyticus regarding the site of collection. None of the 1943 V. parahaemolyticus isolates contained tdh and/or trh. These data provide information for the assessment of exposure to V. parahaemolyticus in oysters consumed in Sao Paulo, State, Brazil.
副溶血性弧菌是一种潜在的致病性细菌，自然存在于世界各地的港湾环境中，常与人类食用生贝类后发生的胃肠炎有关，尤其是生食牡蛎。2006 年 2 月至 2007 年 1 月，在巴西圣保罗州的餐馆、超市、杂货店和海滩小屋采集了 74 份生牡蛎样本，监测了总副溶血性弧菌和致病性副溶血性弧菌的发生情况。副溶血性弧菌的计数根据最可能数（MPN）程序进行。从硫代硫酸盐-柠檬酸盐-胆汁盐-蔗糖（TCBS）琼脂平板上选择 5 到 10 个典型菌落，通过存在种特异性基因 tlh 和毒力基因 tdh 和 trh 的多重 PCR 进行确认。在 100%的样本中检测到副溶血性弧菌。总副溶血性弧菌的密度从 1.78 到 6.04 log(10)（MPN/g）不等，秋季和夏季的密度较高，冬季的密度较低（P < 0.05）。采集地点的副溶血性弧菌密度无统计学差异。1943 株副溶血性弧菌分离株均不含 tdh 和/或 trh。这些数据为评估巴西圣保罗州食用牡蛎中副溶血性弧菌的暴露情况提供了信息。
