Changchai Nuttawee, Saunjit Sudarat
Southeast Asian J Trop Med Public Health. 2014 May;45(3):662-9.
Occurrence, population density and virulence of Vibrio parahaemolyticus and V. vulnificus in 240 retail raw oysters collected monthly between March 2010 and February 2011 from Ang Sila coast, Chon Buri Province, Thailand were determined using most probable number (MPN) multiplex PCR. Multiplex PCR detected V. parahaemolyticus in 219 raw oyster samples, of which 29 samples contained the virulence tdh. MPN values for V. parahaemolyticus and pathogenic strains in most samples ranged from 10 to 10(2) and from 3 to 10 MPN/g, respectively. The presence of V. vulnificus was found in 53 oyster samples in amounts between 10 and 10(2) MPN/g. Of 1,087 V. parahaemolyticus isolates, 14 and 2 isolates carried tdh and virulence trh, respectively but none with both genes. However, none of the presumptive isolates was shown to be V. vulnificus. The detection of pathogenic V. parahaemolyticus and V. vulnificus in raw oysters has rendered high awareness of risk in consumption of raw or undercooked oysters.
2010年3月至2011年2月期间，每月从泰国春武里府阁沙湄海岸采集240份零售生牡蛎样本，运用最大可能数（MPN）多重聚合酶链反应（PCR）测定副溶血性弧菌和创伤弧菌的发生率、种群密度及毒力。多重PCR在219份生牡蛎样本中检测到副溶血性弧菌，其中29份样本含有毒力基因tdh。大多数样本中副溶血性弧菌和致病菌株的MPN值分别为10至10²以及3至10 MPN/g。在53份牡蛎样本中发现了创伤弧菌，含量在10至10² MPN/g之间。在1087株副溶血性弧菌分离株中，分别有14株和2株携带tdh和毒力基因trh，但没有同时携带这两个基因的菌株。然而，所有推定的分离株均未被证明是创伤弧菌。在生牡蛎中检测到致病性副溶血性弧菌和创伤弧菌，这使得人们对食用生的或未煮熟的牡蛎的风险有了高度认识。
