Key Laboratory of the Earth's Deep Interior, Institute of Geology and Geophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China.
Appl Environ Microbiol. 2011 Jan;77(1):323-6. doi: 10.1128/AEM.01476-10. Epub 2010 Nov 5.
A targeted metagenomic approach was applied to investigate magnetotactic bacteria (MTB) within the phylum Nitrospirae in Lake Miyun near Beijing, China. Five fosmids containing rRNA operons were identified. Comparative sequence analysis of a total of 172 kb provided new insights into their genome organization and revealed unexpected subgenomic diversity of uncultivated MTB in the phylum Nitrospirae. In addition, affiliation of two novel MTB with the phylum Nitrospirae was verified by fluorescence in situ hybridization. One of them was morphologically similar to "Candidatus Magnetobacterium bavaricum," but the other differed substantially in cell shape and magnetosome organization from all previously described "Ca. Magnetobacterium bavaricum"-like bacteria.
采用靶向宏基因组学方法研究了中国北京密云湖硝化螺旋菌门中的趋磁细菌（MTB）。鉴定了 5 个包含 rRNA 操纵子的 fosmid。总共 172 kb 的比较序列分析为它们的基因组组织提供了新的见解，并揭示了硝化螺旋菌门中未培养的 MTB 的意外亚基因组多样性。此外，通过荧光原位杂交验证了两种新型 MTB 与硝化螺旋菌门的隶属关系。其中一种在形态上类似于“Candidatus Magnetobacterium bavaricum”，但另一种在细胞形状和磁小体组织上与所有先前描述的“Ca. Magnetobacterium bavaricum”-样细菌有很大的不同。
