Cintra A, Fuxe K, Wikström A C, Visser T, Gustafsson J A
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
Brain Res. 1990 Jan 1;506(1):139-44. doi: 10.1016/0006-8993(90)91210-8.
Neurons containing thyrotropin-releasing hormone (TRH) and glucocorticoid receptor (GR) immunoreactivity (IR) were demonstrated by a two-colour immunoperoxidase method in coronal cryotome sections of the preoptic region and the hypothalamus of the male rat brain. All the TRH-IR neurons (TRH-IR) located in the dorsal hypothalamus - medial and dorsal parvocellular parts of the paraventricular hypothalamic nucleus and the dorsomedial hypothalamic nucleus - and in the anterior periventricular hypothalamic nucleus were strongly GR-IR. The TRH-IR neurons of the medial preoptic area, the perifornical nucleus and the medial tuberal area were mostly weakly GR-IR and some lacked GR-IR. These data indicate a differential regulation of diencephalic TRH-IR neurons by glucocorticoids. They also imply that the inhibitory effect of glucocorticoids on TSH secretion may involve a direct inhibition of TRH synthesis and/or release by a nuclear action in the TRH-IR nerve cells of the paraventricular hypothalamic nucleus projecting to the median eminence.
采用双色免疫过氧化物酶法，在雄性大鼠脑视前区和下丘脑的冠状冷冻切片中，证实了含有促甲状腺激素释放激素（TRH）和糖皮质激素受体（GR）免疫反应性（IR）的神经元。所有位于下丘脑背侧——下丘脑室旁核内侧和背侧小细胞部以及下丘脑背内侧核——以及室周下丘脑前核的TRH免疫反应性神经元（TRH-IR）均有强烈的GR免疫反应性。视前内侧区、穹窿周核和结节内侧区的TRH-IR神经元大多GR免疫反应性较弱，有些则缺乏GR免疫反应性。这些数据表明糖皮质激素对间脑TRH-IR神经元有不同的调节作用。它们还意味着糖皮质激素对促甲状腺激素分泌的抑制作用可能涉及对投射到正中隆起的下丘脑室旁核TRH-IR神经细胞中TRH合成和/或释放的直接抑制，这种抑制是通过核作用实现的。
