Nishiyama T, Kawano H, Tsuruo Y, Maegawa M, Hisano S, Adachi T, Daikoku S, Suzuki M
Brain Res. 1985 Oct 21;345(2):205-18. doi: 10.1016/0006-8993(85)90996-5.
The localization of neurons containing immunoreactive thyrotropin-releasing hormone (TRH) was examined in the hypothalamus of intact, propylthiouracil (PTU)-treated, and colchicine-treated adult rats. In intact animals, immunoreactive TRH neurons were occasionally found in the paraventricular and dorsomedial nuclei, and in the anterior and lateral hypothalamic areas. In PTU-treated animals, the cellular appearance of the hypothalamus with the exception of the paraventricular nucleus was almost similar to that of intact animals. In the paraventricular nucleus, only the cells localized in the periventricular and medial parvocellular subdivisions significantly increased in number and became hypertrophic in comparison with intact animals. The distribution of immunoreactive fibers in the hypothalamus was almost equal among the 3 animal groups with the exception of that in the median eminence, in which the fibers were most densely concentrated in intact animals, and most sparse in PTU-treated rats. The fibers projecting into the median eminence were distinguished into the periventricular and lateral pathways, which are derived from the neurons in the periventricular and medial parvocellular subdivisions of the paraventricular nucleus, respectively. Thus, among immunoreactive TRH neurons in the hypothalamus, only those in the periventricular and medial parvocellular subdivisions of the paraventricular nucleus may be involved in the hypothalamic-hypophysial-thyroid axis.
在完整的、经丙基硫氧嘧啶（PTU）处理的和经秋水仙碱处理的成年大鼠下丘脑中，对含有免疫反应性促甲状腺激素释放激素（TRH）的神经元进行了定位研究。在完整动物中，偶尔可在室旁核和背内侧核以及下丘脑前区和外侧区发现免疫反应性TRH神经元。在PTU处理的动物中，除室旁核外，下丘脑的细胞外观与完整动物几乎相似。在室旁核中，与完整动物相比，仅位于室周和内侧小细胞亚区的细胞数量显著增加且变得肥大。下丘脑免疫反应性纤维的分布在这3组动物中几乎相等，但在正中隆起处除外，其中纤维在完整动物中最密集，在PTU处理的大鼠中最稀疏。投射到正中隆起的纤维可分为室周和外侧通路，它们分别源自室旁核室周和内侧小细胞亚区的神经元。因此，在下丘脑的免疫反应性TRH神经元中，可能只有室旁核室周和内侧小细胞亚区的神经元参与下丘脑 - 垂体 - 甲状腺轴。
