Andrew D, Jayakumar J
Department of Zoology and Cell Biology, University College, London.
Cell Immunol. 1990 Mar;126(1):16-30. doi: 10.1016/0008-8749(90)90297-5.
A set of monoclonal antibodies has been raised to cell surface markers which are either hyperexpressed, or exclusive to activated murine lymphocytes. One antigen is present on all B cells of the Ly1 lineage and defines a novel subpopulation of classical B cells, most of which are activated B cells. In Western blots, this antigen appears as a single glycoprotein of 95 kDa molecular weight but of greater interest, is its expression at high levels on all mature peripheral T cells but on only 30% of thymocytes. This marker is expressed at high levels on all CD4 and CD8 thymocytes, most double-negative (CD4-CD8-) thymocytes, and at low levels on double-positive (CD4+CD8+) thymocytes. It may prove useful as a developmental marker for subdividing the double-positive (CD4+CD8+) and double negative (CD4-CD8-) thymocyte populations. Taken together these results indicate that mab 3.17 recognises a novel lymphocyte antigen expressed on subsets of T and B cells, which is hyperexpressed on lymphocyte activation.
已制备出一组针对细胞表面标志物的单克隆抗体，这些标志物要么过度表达，要么是活化的小鼠淋巴细胞所特有的。一种抗原存在于Ly1谱系的所有B细胞上，并定义了一个新的经典B细胞亚群，其中大多数是活化的B细胞。在蛋白质免疫印迹中，这种抗原表现为一种分子量为95 kDa的单一糖蛋白，但更有趣的是，它在所有成熟外周T细胞上高水平表达，而在仅30%的胸腺细胞上表达。这种标志物在所有CD4和CD8胸腺细胞、大多数双阴性（CD4-CD8-）胸腺细胞上高水平表达，而在双阳性（CD4+CD8+）胸腺细胞上低水平表达。它可能被证明是用于细分双阳性（CD4+CD8+）和双阴性（CD4-CD8-）胸腺细胞群体的有用发育标志物。综合这些结果表明，单克隆抗体3.17识别一种在T和B细胞亚群上表达的新型淋巴细胞抗原，该抗原在淋巴细胞活化时过度表达。
