Serteyn D, Sandersen C, Lejeune J-P, de la Rebière de Pouyade G, Ceusters J, Mouithys-Mickalad A, Niesten A, Fraipont A, van Erck E, Goachet A G, Robert C, Leclerc J L, Votion D-M, Franck T
Equine European Centre of Mont-le-Soie Equine Clinic, Faculty of Veterinary Medicine Centre of Oxygen, University of Liege, Belgium.
Equine Vet J Suppl. 2010 Nov(38):275-9. doi: 10.1111/j.2042-3306.2010.00269.x.
Intense physical exercise can induce the degranulation of neutrophils leading to an increase in plasma concentration of the neutrophil marker enzymes myeloperoxidase (MPO) and elastase (ELT). These enzymes have pro-oxidative and pro-inflammatory properties and may play a role in the exercised-induced muscular damage.
To measure MPO and ELT concentrations in plasma and muscles of endurance horses and to correlate them to the extent of exercise-induced muscular damage.
Seven endurance horses qualified on 120 km races were tested in this study. Neutrophil count, serum creatine kinase (CK), plasmatic and muscular MPO and ELT concentrations were measured before and 2 h after a 120 km endurance race.
The race produced a significant increase of neutrophils, CK, and plasma MPO and ELT levels. A significant correlation was observed between the MPO and ELT values in plasma (r(2) = 0.92, P < 0.01) and in muscles (r(2) = 0.89, P < 0.01) while plasmatic concentrations of MPO and ELT were not significantly correlated to muscular ones. An increase of mean concentrations (± s.e.) of MPO (T0: 9.85 ± 3.9, T1: 228.9 ± 95.9 ng/mg proteins) and ELT (T0: 8.4 ± 2.4, T1: 74.5 ± 39.7 ng/mg proteins) in the muscles were observed after the race. Interestingly, the individual data showed large differences between the horses. Muscular MPO and ELT concentrations were significantly correlated to plasma CK levels. The coefficient of correlation (r(2)) was 0.69 (P < 0.01) for MPO and 0.66 (P < 0.01) for ELT, respectively.
Results underline the possible role of MPO and ELT in exercise-induced muscular damage.
Further studies should investigate the effect of exercise type and intensity, as well as the role of the training state on MPO and ELT involvement in muscular damage. The assessment of the intensity of exercise-induced neutrophilic degranulation may have a potential role in the monitoring of the athletic career.
剧烈体育锻炼可诱导中性粒细胞脱颗粒，导致中性粒细胞标记酶髓过氧化物酶（MPO）和弹性蛋白酶（ELT）的血浆浓度升高。这些酶具有促氧化和促炎特性，可能在运动诱导的肌肉损伤中起作用。
测量耐力赛马血浆和肌肉中MPO和ELT的浓度，并将它们与运动诱导的肌肉损伤程度相关联。
本研究测试了7匹符合120公里耐力赛资格的耐力赛马。在120公里耐力赛之前和之后2小时测量中性粒细胞计数、血清肌酸激酶（CK）、血浆和肌肉中的MPO和ELT浓度。
比赛导致中性粒细胞、CK以及血浆MPO和ELT水平显著升高。血浆中MPO和ELT值之间（r(2) = 0.92，P < 0.01）以及肌肉中MPO和ELT值之间（r(2) = 0.89，P < 0.01）存在显著相关性，而血浆中MPO和ELT浓度与肌肉中的浓度无显著相关性。比赛后观察到肌肉中MPO（T0：9.85 ± 3.9，T1：228.9 ± 95.9 ng/mg蛋白质）和ELT（T0：8.4 ± 2.4，T1：74.5 ± 39.7 ng/mg蛋白质）的平均浓度（±标准误）增加。有趣的是，个体数据显示马匹之间存在很大差异。肌肉中MPO和ELT浓度与血浆CK水平显著相关。MPO的相关系数（r(2)）为0.69（P < 0.01），ELT的相关系数（r(2)）为0.66（P < 0.01）。
结果强调了MPO和ELT在运动诱导的肌肉损伤中的可能作用。
进一步的研究应调查运动类型和强度的影响，以及训练状态在MPO和ELT参与肌肉损伤中的作用。评估运动诱导的嗜中性粒细胞脱颗粒强度可能在运动员职业生涯监测中具有潜在作用。
