Cywinska A, Gorecka R, Szarska E, Witkowski L, Dziekan P, Schollenberger A
Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
Equine Vet J Suppl. 2010 Nov(38):23-7. doi: 10.1111/j.2042-3306.2010.00280.x.
Changes in serum levels of acute phase proteins (APPs) reflect the acute phase reaction, a rapid and nonspecific response to any tissue damage. Serum amyloid A (SAA) is the main APP in horses, which increases in various diseases, surgical injuries and after long distance endurance rides; however, this nonspecific parameter has not been investigated as an indicator of subclinical disorders, which may result in elimination during endurance competitions.
To evaluate the serum concentration of SAA as a potential indicator for the status of horses prepared for long distance endurance rides (120 and 160 km).
Twenty Arabian horses were tested and 12 were eliminated during the ride and 8 completed the distances. Routine haematological and biochemical tests and measurement of serum concentrations of SAA were carried out before and after the competition. Results were compared using the Mann Whitney U test.
Before the competition all haematological and biochemical parameters varied within reference ranges with no differences between the eliminated horses and the ones that successfully finished the competition. After the rides creatine phosphokinase activity and neutrophil: lymphocyte ratio reflecting exercise-induced leukogram changes increased (P < 0.05) in both groups. Before the competition, the concentration of SAA remained within reference ranges; however, it was significantly (P < 0.05) lower in horses that successfully finished the competition than in eliminated ones (411.7 ± 144 ng/ml vs. 5809.5 ± 2242.7 ng/ml). After the ride SAA levels increased (P < 0.05) and were similar in both groups (13,833.8 ± 1354.3 ng/ml and 12,831.2 ± 1317.6 ng/ml).
Serum SAA level was the only laboratory parameter that indicated most (66.6%) of the eliminated horses before entering the competition. None of the horses with SAA level higher than 1000 ng/ml completed the distance. Thus, it may be postulated that serum SAA concentration may indicate a poor status of a horse, resulting in elimination during a competition.
急性期蛋白（APPs）血清水平的变化反映了急性期反应，这是对任何组织损伤的快速且非特异性反应。血清淀粉样蛋白A（SAA）是马体内主要的急性期蛋白，在各种疾病、手术损伤以及长途耐力赛后会升高；然而，这一非特异性参数尚未作为亚临床疾病的指标进行研究，而亚临床疾病可能导致马匹在耐力赛中被淘汰。
评估血清SAA浓度作为准备参加长途耐力赛（120公里和160公里）马匹状态的潜在指标。
对20匹阿拉伯马进行测试，其中12匹在比赛中被淘汰，8匹完成了赛程。在比赛前后进行常规血液学和生化检测以及血清SAA浓度测定。结果采用曼-惠特尼U检验进行比较。
比赛前，所有血液学和生化参数均在参考范围内，被淘汰的马匹与成功完成比赛的马匹之间无差异。赛后，两组中反映运动诱导白细胞图谱变化的肌酸磷酸激酶活性和中性粒细胞：淋巴细胞比值均升高（P < 0.05）。比赛前，SAA浓度保持在参考范围内；然而，成功完成比赛的马匹中SAA浓度显著（P < 0.05）低于被淘汰的马匹（411.7 ± 144纳克/毫升对5809.5 ± 2242.7纳克/毫升）。赛后SAA水平升高（P < 0.05），两组相似（13,833.8 ± 1354.3纳克/毫升和12,831.2 ± 1317.6纳克/毫升）。
血清SAA水平是唯一能在比赛前表明大多数（66.6%）被淘汰马匹状态的实验室参数。SAA水平高于1000纳克/毫升的马匹无一完成赛程。因此，可以推测血清SAA浓度可能表明马匹状态不佳，导致在比赛中被淘汰。
