MUC16作为上皮内生的敏感且特异的标志物。
MUC16 as a sensitive and specific marker for epithelial downgrowth.
作者信息
Pai Vicky C, Glasgow Ben J
机构信息
Department of Ophthalmology, Jules Stein Eye Institute, University of California at Los Angeles School of Medicine, Los Angeles, CA 90095-7000, USA.
出版信息
Arch Ophthalmol. 2010 Nov;128(11):1407-12. doi: 10.1001/archophthalmol.2010.268.
OBJECTIVE
To compare immunohistochemical results of cytokeratin AE1/AE3, the traditional favored marker, with MUC16 and cytokeratin 19 for diagnostic sensitivity and specificity in epithelial downgrowth and control corneas.
METHODS
Immunohistochemical analysis was performed in 5 cases of epithelial downgrowth and 5 control specimens for MUC16, cytokeratin AE1/AE3, and cytokeratin 19 using the immunoperoxidase method. The mean percentages of reactive cells on the epithelium and endothelium were compared for each antibody using the Wilcoxon rank sum test. The sensitivity and specificity for each marker were compared.
RESULTS
All 3 antibodies showed high sensitivity (100%) in identifying epithelial downgrowth. However, the specificity was greatest for MUC 16 (100%) compared with cytokeratin 19 (80%) and cytokeratin AE1/AE3 (0%). None of the endothelial cells in any case showed reactivity to anti-MUC16 compared with anti-cytokeratin AE1/AE3 (mean [SD], 0.0% [0.0%] vs 17.4% [10.4%]; P = .008). Cytokeratin 19 was positive in every case of epithelial downgrowth but showed focal staining of the endothelium (3.4% of cells) in 1 control.
CONCLUSIONS
Antibodies for MUC16, cytokeratin AE1/AE3, and cytokeratin 19 are equally sensitive for downgrowth. However, anti-MUC16 showed superior specificity compared with anti-cytokeratin 19 or anti-cytokeratin AE1/AE3 in this study.
目的
比较传统常用标志物细胞角蛋白AE1/AE3与MUC16和细胞角蛋白19在诊断上皮内生和对照角膜中的免疫组化结果的诊断敏感性和特异性。
方法
采用免疫过氧化物酶法对5例上皮内生病例和5例对照标本进行MUC16、细胞角蛋白AE1/AE3和细胞角蛋白19的免疫组化分析。使用Wilcoxon秩和检验比较每种抗体在上皮和内皮上反应性细胞的平均百分比。比较每种标志物的敏感性和特异性。
结果
所有3种抗体在识别上皮内生方面均显示出高敏感性（100%）。然而，与细胞角蛋白19（80%）和细胞角蛋白AE1/AE3（0%）相比，MUC16的特异性最高（100%）。与抗细胞角蛋白AE1/AE3相比，任何病例中的内皮细胞均未显示对抗MUC16的反应性（平均值[标准差]，0.0%[0.0%]对17.4%[10.4%]；P = 0.008）。细胞角蛋白19在每例上皮内生病例中均为阳性，但在1例对照中显示内皮细胞有局灶性染色（3.4%的细胞）。
结论
MUC16、细胞角蛋白AE1/AE3和细胞角蛋白19的抗体在上皮内生方面同样敏感。然而，在本研究中，抗MUC16与抗细胞角蛋白19或抗细胞角蛋白AE1/AE3相比显示出更高的特异性。
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