Sadiq Soban, Nagi Abdul Hannan, Shahzad Muhammad, Zia Azam
Department of Pharmacology, University of Health Sciences, Khayaban-e-Jamia Punjab, Lahore, Pakistan.
Saudi J Kidney Dis Transpl. 2010 Nov;21(6):1058-65.
To assess the effect of aqueous extract of Carum carvi seeds in experimentally induced diabetic nephropathy (DN) in rodents, we studied 48 adult male Wistar rats divided into 4 groups: normal controls (group A), diabetes positive control (group B), and experimental (groups C and D). They received Carum carvi extract as a renoprotective agent. Rats having fasting blood glucose levels over 280 mg/dL were included in this study. Group C rats received STZ (60 mg/kg) and aqueous extract of Carum carvi at 30 mg/kg of body weights. On the other hand group D rats received STZ (60 mg/kg) and aqueous extract of Carum carvi at 60 mg/kg of body weight. Blood samples were collected on the 60 th day, and kidneys were also extracted for examination. The diabetic group rats showed a variable increase in the serum levels of glucose, urea, creatinine, total urinary protein and microalbuminuric levels. Body weight decreased and urine volume increased in the diabetic groups. 30 mg/kg body weight of Carum carvi dose decreased the levels of these parameters in rats. On the other hand, 60 mg/kg body weight of Carum carvi dose significantly decreased the levels of the biochemical parameters. The morphological examination of group C rats showed no changes whereas the rats in group D showed moderate changes. Carum carvi constituents, especially flavonoids and carvone have strong anti-oxidant activity, which provides reno-protection against diabetes and its complications. In conclusion, high dose of Carum carvi aqueous seeds extract (60 mg/kg) showed reno-protection against STZ induced dia-betic nephropathy in rats.
为评估葛缕子种子水提取物对实验性诱导的啮齿动物糖尿病肾病（DN）的影响，我们研究了48只成年雄性Wistar大鼠，将其分为4组：正常对照组（A组）、糖尿病阳性对照组（B组）和实验组（C组和D组）。它们接受葛缕子提取物作为肾脏保护剂。本研究纳入空腹血糖水平超过280 mg/dL的大鼠。C组大鼠接受链脲佐菌素（STZ，60 mg/kg）和体重30 mg/kg的葛缕子水提取物。另一方面，D组大鼠接受链脲佐菌素（60 mg/kg）和体重60 mg/kg的葛缕子水提取物。在第60天采集血样，并提取肾脏进行检查。糖尿病组大鼠的血糖、尿素、肌酐、总尿蛋白和微量白蛋白尿水平有不同程度升高。糖尿病组大鼠体重下降，尿量增加。体重30 mg/kg的葛缕子剂量可降低大鼠这些参数的水平。另一方面，体重60 mg/kg的葛缕子剂量可显著降低生化参数水平。C组大鼠的形态学检查未显示变化，而D组大鼠显示中度变化。葛缕子成分，尤其是黄酮类化合物和香芹酮具有强大的抗氧化活性，可对糖尿病及其并发症提供肾脏保护。总之，高剂量的葛缕子种子水提取物（60 mg/kg）对链脲佐菌素诱导的大鼠糖尿病肾病具有肾脏保护作用。
