Institute of Analytical Chemistry, University of Leipzig, Linnéstr. 3, 04103, Leipzig, Germany.
Lab Chip. 2011 Jan 21;11(2):309-14. doi: 10.1039/c0lc00347f. Epub 2010 Nov 8.
In this work, a microfluidic free-flow electrophoresis device with a novel approach for preventing gas bubbles from entering the separation area is presented. This is achieved by integrating partitioning bars to reduce the channel depth between electrode channels and separation chamber in order to obtain electrical contact and simultaneously prevent bubbles from entering the separation area. The three-layer sandwich chip features a reusable carrier plate with integrated ports for fluidic connection combined with a softlithographically cast microfluidic PDMS layer and a sealing glass slide. This design allows for a straightforward and rapid chip prototyping process. The performance of the device is demonstrated by free-flow zone electrophoretic separations of fluorescent dye mixtures as well as by the separation of labeled amines and amino acids with separation voltages up to 297 V.
本工作提出了一种微流控自由流电泳装置，该装置采用了一种新颖的方法来防止气泡进入分离区。通过集成分隔条来减小电极通道和分离室之间的通道深度，从而实现这一点，以获得电接触并同时防止气泡进入分离区。该三层三明治芯片具有带有集成端口的可重复使用的载玻片，用于流体连接，结合了软光刻铸造的微流控 PDMS 层和密封玻璃载玻片。这种设计允许进行简单快捷的芯片原型制作过程。该设备的性能通过荧光染料混合物的自由流动区电泳分离以及通过分离电压高达 297 V 的标记胺和氨基酸的分离来证明。
