CSIRO Plant Industry, Canberra, Australia.
Theor Appl Genet. 2011 Mar;122(4):735-44. doi: 10.1007/s00122-010-1482-7.
The stem rust resistance gene Sr2 has provided broad-spectrum protection against stem rust (Puccinia graminis Pers. f. sp. tritici) since its wide spread deployment in wheat from the 1940s. Because Sr2 confers partial resistance which is difficult to select under field conditions, a DNA marker is desirable that accurately predicts Sr2 in diverse wheat germplasm. Using DNA sequence derived from the vicinity of the Sr2 locus, we developed a cleaved amplified polymorphic sequence (CAPS) marker that is associated with the presence or absence of the gene in 115 of 122 (95%) diverse wheat lines. The marker genotype predicted the absence of the gene in 100% of lines which were considered to lack Sr2. Discrepancies were observed in lines that were predicted to carry Sr2 but failed to show the CAPS marker. Given the high level of accuracy observed, the marker provides breeders with a selection tool for one of the most important disease resistance genes of wheat.
自 20 世纪 40 年代以来，茎锈病抗性基因 Sr2 在小麦中的广泛应用为其提供了广谱的茎锈病抗性。由于 Sr2 提供的是部分抗性，在田间条件下很难选择，因此需要一个能够准确预测不同小麦种质中 Sr2 的 DNA 标记。利用来自 Sr2 基因座附近的 DNA 序列，我们开发了一种与基因存在或不存在相关的切割扩增多态性序列（CAPS）标记，该标记与 122 个不同小麦品系中的 115 个（95%）有关。标记基因型在 100%被认为缺乏 Sr2 的品系中预测基因的不存在。在预测携带 Sr2 但未能显示 CAPS 标记的品系中观察到差异。鉴于观察到的高度准确性，该标记为育种者提供了一种选择工具，用于小麦最重要的抗病基因之一。
