Diéguez María José, López Micaela, Altieri Emiliano, Pergolesi María Fernanda, Dabove Marisol Alicia, Cuyeu Alba Romina, Justus Nadia, Kandus Mariana, Ingala Lorena, Sacco Francisco
Instituto de Genética "Ewald A. Favret", Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria CC25 (1712), Castelar B1712, Argentina.
Instituto de Recursos Biológicos, Centro de Investigación de Recursos Naturales, Instituto Nacional de Tecnología Agropecuaria CC25 (1712), Castelar B1712, Argentina.
Biology (Basel). 2021 Nov 12;10(11):1168. doi: 10.3390/biology10111168.
Leaf rust is one of the most significant diseases of wheat worldwide. In Argentina, it is one of the main reasons for variety replacement that becomes susceptible after large-scale use. Some varieties showed durable resistance to this disease, including Buck Manantial and Sinvalocho MA. RILs (Recombinant Inbred Lines) were developed for each of these varieties and used in genetics studies to identify components of resistance, both in greenhouse inoculations using leaf rust races, and in field evaluations under natural population infections. In Buck Manantial, the APR gene was associated with resistance in field tests. In crosses involving Sinvalocho MA, four genes were previously identified and associated with resistance in field testing: APR (Adult Plant Resistance) gene , the APR genetic system + and the ASR (All Stage Resistance) gene . Using backcrosses, was introgressed in four commercial susceptible varieties and , + and were simultaneously introgressed in three susceptible commercial varieties. The use of molecular markers for recurrent parent background selection allowed us to select resistant lines with more than 80% similarity to commercial varieties. Additionally, progress towards positional cloning of the genetic system + for leaf rust APR is reported.
叶锈病是全球小麦最重要的病害之一。在阿根廷，它是大规模使用后变得易感的品种被替换的主要原因之一。一些品种对这种病害表现出持久抗性，包括巴克马纳蒂亚尔和辛瓦洛乔MA。针对这些品种分别培育了重组自交系（RILs），并用于遗传学研究，以确定抗性成分，包括在使用叶锈菌生理小种的温室接种试验以及自然群体感染下的田间评估中。在巴克马纳蒂亚尔中，成株抗性（APR）基因在田间试验中与抗性相关。在涉及辛瓦洛乔MA的杂交中，先前已鉴定出四个基因，并在田间试验中与抗性相关：成株抗性（APR）基因、成株抗性遗传系统+以及全生育期抗性（ASR）基因。通过回交，将导入四个感病商业品种，同时将、+和导入三个感病商业品种。利用分子标记进行轮回亲本背景选择，使我们能够选择出与商业品种相似度超过80%的抗性品系。此外，还报告了在叶锈病成株抗性遗传系统+的定位克隆方面取得的进展。
