Carel J C, Schreiber R D, Falqui L, Lacy P E
Department of Pathology, Washington University School of Medicine, Saint Louis, MO 63110.
Proc Natl Acad Sci U S A. 1990 Feb;87(4):1591-5. doi: 10.1073/pnas.87.4.1591.
Culture of rat islets of Langerhans for 1 week at 37 degrees C with recombinant transforming growth factor beta prolonged the survival of islet xenografts transplanted into diabetic mouse recipients. Treatment of diabetic recipients with a neutralizing monoclonal antibody to murine interferon gamma did not affect the survival of islet xenografts cultured 7 days in control medium. However, treatment of donor islets with transforming growth factor beta in combination with treatment of diabetic recipients with interferon gamma antibody produced a 75% survival of the islet xenografts at 100 days. Fifty percent of the recipients who had accepted their graft for more than 100 days were immune unresponsive to a transplant of freshly isolated islets from the same donor strain.
将大鼠胰岛在37摄氏度下用重组转化生长因子β培养1周，可延长移植到糖尿病小鼠受体中的胰岛异种移植物的存活时间。用抗小鼠干扰素γ的中和单克隆抗体治疗糖尿病受体，对在对照培养基中培养7天的胰岛异种移植物的存活没有影响。然而，用转化生长因子β处理供体胰岛并结合用干扰素γ抗体治疗糖尿病受体，在100天时胰岛异种移植物的存活率达到75%。接受移植物超过100天的受体中，有50%对来自同一供体品系的新鲜分离胰岛移植无免疫反应。
