Redchuk T A, Korotkevich N V, Kaberniuk A A, Oleĭnik E S, Labyntsev A Iu, Romaniuk S I, Kolibo D V, Busol V A, Komisarenko S V
Tsitol Genet. 2010 Sep-Oct;44(5):23-30.
Antibody responses to purified protein derivate PPD of tuberculin and to antigens MPB63 and MPB83 of Mycobacterium bovis were determined in bovine herd (94 adult animals). Statistical approach based on approximation by multiple Gaussians with Levenberg-Marquardt algorithm for analysis of antibody level distribution against antigens examined was provided. Our results confirm that indirect ELISA with recombinant MPB83 and MPB63 as well as conventional PPD could be used for test-systems development for detection of cow tuberculosis infection at the herd level.
在一个牛群（94头成年动物）中测定了对结核菌素纯蛋白衍生物PPD以及对牛分枝杆菌抗原MPB63和MPB83的抗体反应。提供了基于多个高斯近似和Levenberg-Marquardt算法的统计方法，用于分析针对所检测抗原的抗体水平分布。我们的结果证实，以重组MPB83和MPB63以及传统PPD作为抗原的间接ELISA可用于开发检测牛群中牛结核病感染的检测系统。
