Cho Young-Keol, Jung You-Sun, Foley Brian T
Department of Microbiology, University of Ulsan College of Medicine, Seoul, South Korea.
AIDS Res Hum Retroviruses. 2011 Jun;27(6):613-21. doi: 10.1089/AID.2010.0174. Epub 2011 Jan 5.
There was an outbreak of HIV-1 transmission among 20 out of 122 Korean hemophiliacs from 1990. We assessed the genetic relationships among HIV-1 viruses found in three cash-paid plasma donors whose preseroconversion plasma was used to produce Korean-made clotting factor, 20 hemophiliacs infected with HIV-1 in Korea, three hemophiliacs infected with HIV-1 from clotting factor manufactured outside Korea, and 71 local control patients infected with the Korean subclade of HIV-1 subtype B (KSB). Full-length pol gene sequences (2841 bp) of viruses from frozen stored serum, samples obtained 1-3 years after diagnosis, were amplified by RT-PCR and sequenced by direct DNA sequencing. Phylogenetic and signature pattern analyses were used to investigate the relationships among the sequences. Donors O and P were associated with two clusters, of 8 and 12 hemophiliacs, respectively, which were demarcated from the 71 KSB-infected local control patients and donor R. These data confirm that HIV-1 transmission to 20 hemophiliacs occurred through infusion of Korean-made clotting factor.
自1990年起，122名韩国血友病患者中有20人发生了HIV-1传播。我们评估了在三名现金支付血浆捐献者中发现的HIV-1病毒之间的遗传关系，这些捐献者的血清转化前血浆被用于生产韩国制造的凝血因子；20名在韩国感染HIV-1的血友病患者；三名因使用韩国境外制造的凝血因子而感染HIV-1的血友病患者；以及71名感染HIV-1 B亚型韩国分支（KSB）的本地对照患者。通过RT-PCR扩增来自冷冻保存血清（诊断后1 - 3年获取的样本）中病毒的全长pol基因序列（2841 bp），并通过直接DNA测序进行测序。采用系统发育和特征模式分析来研究序列之间的关系。捐献者O和P分别与两个集群相关，这两个集群分别有8名和12名血友病患者，它们与71名感染KSB的本地对照患者及捐献者R区分开来。这些数据证实，20名血友病患者的HIV-1传播是通过输注韩国制造的凝血因子发生的。
