鱼类基因定位在牛（Bos taurus L.）中仍未过时。

FISH mapping in cattle (Bos taurus L.) is not yet out of fashion.

机构信息

Department of Animal Science, Milan University, Milan, Italy.

出版信息

J Appl Genet. 2010;51(4):497-9. doi: 10.1007/BF03208879.

DOI:10.1007/BF03208879

Abstract

Physical mapping of genes by fluorescence in situ hybridization (FISH) seems to be out of fashion in species whose assembled genome sequences are available. However, in this work we evidence the existence of errors in gene location in the Btau_4.0 assembly. We show that DFNA5 and CHCHD6 genes are located on BTA4 and BTA22, respectively, instead of BTA10 and BTA3, as displayed by Btau_4.0. This report emphasizes the need to verify the data on physical localization of genes in the cattle genome (at least by taking into account comparative data reported in available papers) and the need to improve the cattle genome assembly. Our results indicate that FISH mapping in cattle is still useful.

摘要

通过荧光原位杂交（FISH）进行基因的物理图谱绘制在那些已经有完整基因组序列的物种中似乎已经过时了。然而，在这项工作中，我们证明了 Btau_4.0 组装中存在基因位置错误。我们表明，DFNA5 和 CHCHD6 基因分别位于 BTA4 和 BTA22 上，而不是 BTA10 和 BTA3 上，正如 Btau_4.0 所显示的。这一报告强调了需要验证牛基因组中基因的物理定位数据（至少要考虑到现有文献中报道的比较数据），并需要改进牛基因组组装。我们的结果表明，在牛中进行 FISH 图谱绘制仍然是有用的。