• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于全基因组分析，LY6K 是膀胱癌的一个新的分子靶标。

LY6K is a novel molecular target in bladder cancer on basis of integrate genome-wide profiling.

机构信息

Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan.

出版信息

Br J Cancer. 2011 Jan 18;104(2):376-86. doi: 10.1038/sj.bjc.6605990. Epub 2010 Nov 9.

DOI:10.1038/sj.bjc.6605990
PMID:21063397
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3031884/
Abstract

BACKGROUND

The aim of this study is to find a novel molecular target based on chromosomal alteration and array-based gene expression analyses in bladder cancer (BC). We investigated a cancer testis antigen, LY6K, which is located on chromosome 8q24.3.

METHODS

Five BC cell lines were subjected to high-resolution array-comparative genomic hybridisation with 244 000 probes. The expression levels of LY6K mRNA were evaluated in BC cell lines and clinical BC specimens by real-time reverse transcription-PCR. The cell lines were subjected to fluorescence in situ hybridisation of LY6K. Cell viability was evaluated by cell growth, wound healing, and matrigel invasion assays.

RESULTS

Typical gained loci (P<0.0001) at 6p21.33-p21.32, 8q24.3, 9q34.13, 11q13.1-q14.1, 12q13.12-q13.13, 16p13.3, and 20q11.21-q13.33 were observed in all of the cell lines. We focused on 8q24.3 locus where LY6K gene harbours, and it was the top upregulated one in the gene profile from the BC cell line. LY6K mRNA expression was significantly higher in 91 BCs than in 37 normal bladder epitheliums (P<0.0001). Fluorescence in situ hybridisation validated that the high LY6K mRNA expression was due to gene amplification in the region where the gene harbours. Cell viability assays demonstrated that significant inhibitions of cell growth, migration, and invasion occured in LY6K knock down BC cell lines; converse phenomena were observed in a stable LY6K transfectant; and LY6K knockdown of the transfectant retrieved the original phenotype from the LY6K transfectant.

CONCLUSION

Upregulation of the oncogenic LY6K gene located on the gained locus at 8q24.3 may contribute BC development.

摘要

背景

本研究旨在通过染色体改变和基于阵列的基因表达分析在膀胱癌（BC）中找到一个新的分子靶标。我们研究了位于 8q24.3 上的癌症睾丸抗原 LY6K。

方法

对 5 个 BC 细胞系进行了 244000 个探针的高分辨率阵列比较基因组杂交。通过实时逆转录-PCR 评估了 LY6K mRNA 在 BC 细胞系和临床 BC 标本中的表达水平。对 LY6K 的细胞系进行了荧光原位杂交。通过细胞生长、伤口愈合和基质胶侵袭测定评估细胞活力。

结果

在所有细胞系中均观察到典型的获得性位点（P<0.0001），位于 6p21.33-p21.32、8q24.3、9q34.13、11q13.1-q14.1、12q13.12-q13.13、16p13.3 和 20q11.21-q13.33。我们专注于 8q24.3 基因座，该基因座是 BC 细胞系基因谱中上调最显著的基因座。91 例 BC 中 LY6K mRNA 表达明显高于 37 例正常膀胱上皮（P<0.0001）。荧光原位杂交验证了高 LY6K mRNA 表达是由于该基因所在区域的基因扩增。细胞活力测定表明，在 LY6K 敲低的 BC 细胞系中，细胞生长、迁移和侵袭明显受到抑制；在稳定转染的 LY6K 中观察到相反的现象；LY6K 敲低的转染体从 LY6K 转染体中恢复了原始表型。

结论

位于 8q24.3 获得性基因座上的致癌 LY6K 基因的上调可能有助于 BC 的发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/bb20396d2b4a/6605990f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/98c2aba379fa/6605990f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/bfd7a2f6249f/6605990f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/9f8f2430c629/6605990f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/9c8eed9f3528/6605990f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/f78ff3afbc5e/6605990f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/bb20396d2b4a/6605990f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/98c2aba379fa/6605990f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/bfd7a2f6249f/6605990f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/9f8f2430c629/6605990f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/9c8eed9f3528/6605990f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/f78ff3afbc5e/6605990f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3ff/3031884/bb20396d2b4a/6605990f6.jpg

相似文献

1
LY6K is a novel molecular target in bladder cancer on basis of integrate genome-wide profiling.基于全基因组分析，LY6K 是膀胱癌的一个新的分子靶标。
Br J Cancer. 2011 Jan 18;104(2):376-86. doi: 10.1038/sj.bjc.6605990. Epub 2010 Nov 9.
2
CpG hypermethylation of collagen type I alpha 2 contributes to proliferation and migration activity of human bladder cancer.I型胶原α2的CpG高甲基化促进人膀胱癌的增殖和迁移活性。
Int J Oncol. 2009 Jun;34(6):1593-602. doi: 10.3892/ijo_00000289.
3
MiRNA-500a-3p inhibits cell proliferation and invasion by targeting lymphocyte antigen 6 complex locus K (LY6K) in human non-small cell lung cancer.miRNA-500a-3p 通过靶向淋巴细胞抗原 6 复合体基因座 K（LY6K）抑制人非小细胞肺癌细胞的增殖和侵袭。
Neoplasma. 2018 Sep 19;65(5):673-682. doi: 10.4149/neo_2018_170516N355. Epub 2018 Sep 4.
4
MiR-133a induces apoptosis through direct regulation of GSTP1 in bladder cancer cell lines.miR-133a 通过直接调控 GSTP1 诱导膀胱癌细胞系凋亡。
Urol Oncol. 2013 Jan;31(1):115-23. doi: 10.1016/j.urolonc.2010.09.017. Epub 2011 Mar 10.
5
The regulatory mechanism of the LY6K gene expression in human breast cancer cells.LY6K 基因在人乳腺癌细胞中表达的调控机制。
J Biol Chem. 2012 Nov 9;287(46):38889-900. doi: 10.1074/jbc.M112.394270. Epub 2012 Sep 17.
6
miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1 expression in bladder cancer.miR-145 和 miR-133a 作为肿瘤抑制因子发挥作用，直接调控膀胱癌中 FSCN1 的表达。
Br J Cancer. 2010 Mar 2;102(5):883-91. doi: 10.1038/sj.bjc.6605570. Epub 2010 Feb 16.
7
Functional role of LASP1 in cell viability and its regulation by microRNAs in bladder cancer.LASP1 在细胞活力中的功能作用及其在膀胱癌中受 microRNAs 的调控。
Urol Oncol. 2012 Jul-Aug;30(4):434-43. doi: 10.1016/j.urolonc.2010.05.008. Epub 2010 Sep 16.
8
Identification of differentially expressed genes in human bladder cancer through genome-wide gene expression profiling.通过全基因组基因表达谱分析鉴定人类膀胱癌中差异表达的基因。
Oncol Rep. 2006 Sep;16(3):521-31.
9
Ly6E/K Signaling to TGFβ Promotes Breast Cancer Progression, Immune Escape, and Drug Resistance.Ly6E/K向转化生长因子β的信号传导促进乳腺癌进展、免疫逃逸和耐药性。
Cancer Res. 2016 Jun 1;76(11):3376-86. doi: 10.1158/0008-5472.CAN-15-2654. Epub 2016 Apr 11.
10
Cancer-testis antigen lymphocyte antigen 6 complex locus K is a serologic biomarker and a therapeutic target for lung and esophageal carcinomas.癌-睾丸抗原淋巴细胞抗原6复合体基因座K是一种血清学生物标志物，也是肺癌和食管癌的治疗靶点。
Cancer Res. 2007 Dec 15;67(24):11601-11. doi: 10.1158/0008-5472.CAN-07-3243.

引用本文的文献

1
Comprehensive Mendelian randomization and colocalization analysis of plasma proteomics to identify new therapeutic targets for bladder cancer.血浆蛋白质组学的综合孟德尔随机化和共定位分析以鉴定膀胱癌的新治疗靶点。
J Cancer. 2025 Jul 11;16(10):3163-3179. doi: 10.7150/jca.116402. eCollection 2025.
2
A Phase 2 Study of S-588410 Maintenance Monotherapy for Platinum-Treated Advanced or Metastatic Urothelial Carcinoma.S-588410维持单药治疗铂类治疗的晚期或转移性尿路上皮癌的2期研究。
Bladder Cancer. 2022 Jun 3;8(2):179-192. doi: 10.3233/BLC-211592. eCollection 2022.
3
Silencing LY6D Expression Inhibits Colon Cancer in Xenograft Mice and Regulates Colon Cancer Stem Cells' Proliferation, Stemness, Invasion, and Apoptosis via the MAPK Pathway.

本文引用的文献

1
Metastatic effect of LY-6K gene in breast cancer cells.LY-6K基因在乳腺癌细胞中的转移作用。
Int J Oncol. 2009 Sep;35(3):601-7. doi: 10.3892/ijo_00000371.
2
Cancer statistics, 2009.2009年癌症统计数据。
CA Cancer J Clin. 2009 Jul-Aug;59(4):225-49. doi: 10.3322/caac.20006. Epub 2009 May 27.
3
Exploring contrary trends in bladder cancer incidence, mortality and survival: implications for research and cancer control.探讨膀胱癌发病率、死亡率和生存率的相反趋势：对研究和癌症控制的启示。
沉默 LY6D 表达通过 MAPK 通路抑制异种移植瘤小鼠的结肠癌并调节结肠癌干细胞的增殖、干性、侵袭和凋亡。
Molecules. 2023 Nov 25;28(23):7776. doi: 10.3390/molecules28237776.
4
Genome instability-derived genes as a novel prognostic signature for lung adenocarcinoma.基因组不稳定衍生基因作为肺腺癌的一种新型预后标志物
Front Cell Dev Biol. 2023 Aug 16;11:1224069. doi: 10.3389/fcell.2023.1224069. eCollection 2023.
5
Lymphocyte antigen 6K signaling to aurora kinase promotes advancement of the cell cycle and the growth of cancer cells, which is inhibited by LY6K-NSC243928 interaction.淋巴细胞抗原 6K 向极光激酶发出信号，促进细胞周期的推进和癌细胞的生长，而 LY6K-NSC243928 相互作用则抑制了这一过程。
Cancer Lett. 2023 Apr 1;558:216094. doi: 10.1016/j.canlet.2023.216094. Epub 2023 Feb 16.
6
Establishing a Novel Gene Signature Related to Histone Modifications for Predicting Prognosis in Lung Adenocarcinoma.建立一种与组蛋白修饰相关的新型基因特征用于预测肺腺癌预后
J Oncol. 2022 Sep 23;2022:8802573. doi: 10.1155/2022/8802573. eCollection 2022.
7
A narrative review of the emerging role of lymphocyte antigen 6 complex locus K in cancer: from basic research to clinical practice.淋巴细胞抗原6复合体基因座K在癌症中新兴作用的叙述性综述：从基础研究到临床实践
Ann Transl Med. 2022 Jan;10(1):26. doi: 10.21037/atm-21-5831.
8
Down-regulation of ZNF252P-AS1 alleviates ovarian cancer progression by binding miR-324-3p to downregulate LY6K.下调 ZNF252P-AS1 通过与 miR-324-3p 结合来下调 LY6K，从而减轻卵巢癌的进展。
J Ovarian Res. 2022 Jan 3;15(1):1. doi: 10.1186/s13048-021-00933-7.
9
Differential alternative RNA splicing and transcription events between tumors from African American and White patients in The Cancer Genome Atlas.癌症基因组图谱中非洲裔美国人和白人患者肿瘤之间的差异选择性 RNA 剪接和转录事件。
Genomics. 2021 May;113(3):1234-1246. doi: 10.1016/j.ygeno.2021.02.020. Epub 2021 Mar 8.
10
RNAseq expression patterns of canine invasive urothelial carcinoma reveal two distinct tumor clusters and shared regions of dysregulation with human bladder tumors.犬浸润性尿路上皮癌的 RNAseq 表达模式揭示了两个不同的肿瘤簇，并与人类膀胱癌存在共同失调区域。
BMC Cancer. 2020 Mar 24;20(1):251. doi: 10.1186/s12885-020-06737-0.
Intern Med J. 2010 May;40(5):357-62. doi: 10.1111/j.1445-5994.2009.01980.x.
4
Vaccination with multiple peptides derived from novel cancer-testis antigens can induce specific T-cell responses and clinical responses in advanced esophageal cancer.用源自新型癌-睾丸抗原的多种肽进行疫苗接种可在晚期食管癌中诱导特异性T细胞反应和临床反应。
Cancer Sci. 2009 Aug;100(8):1502-9. doi: 10.1111/j.1349-7006.2009.01200.x. Epub 2009 May 14.
5
Tubulin: a target for antineoplastic drugs into the cancer cells but also in the peripheral nervous system.微管蛋白：抗肿瘤药物的一个靶点，不仅存在于癌细胞中，也存在于外周神经系统中。
Curr Med Chem. 2009;16(11):1315-24. doi: 10.2174/092986709787846488.
6
Kinesin motor proteins as targets for cancer therapy.驱动蛋白作为癌症治疗的靶点。
Cancer Metastasis Rev. 2009 Jun;28(1-2):197-208. doi: 10.1007/s10555-009-9185-8.
7
The galanin signaling cascade is a candidate pathway regulating oncogenesis in human squamous cell carcinoma.甘丙肽信号级联反应是调节人类鳞状细胞癌肿瘤发生的一个候选途径。
Genes Chromosomes Cancer. 2009 Feb;48(2):132-42. doi: 10.1002/gcc.20626.
8
A Joinpoint regression analysis of long-term trends in cancer mortality in Japan (1958-2004).日本癌症死亡率长期趋势的Joinpoint回归分析（1958 - 2004年）。
Int J Cancer. 2009 Jan 15;124(2):443-8. doi: 10.1002/ijc.23911.
9
Cancer-testis antigen lymphocyte antigen 6 complex locus K is a serologic biomarker and a therapeutic target for lung and esophageal carcinomas.癌-睾丸抗原淋巴细胞抗原6复合体基因座K是一种血清学生物标志物，也是肺癌和食管癌的治疗靶点。
Cancer Res. 2007 Dec 15;67(24):11601-11. doi: 10.1158/0008-5472.CAN-07-3243.
10
Inactivation of the Rb pathway and overexpression of both isoforms of E2F3 are obligate events in bladder tumours with 6p22 amplification.在伴有6p22扩增的膀胱肿瘤中，Rb通路失活以及E2F3两种亚型的过表达是必然发生的事件。
Oncogene. 2008 Apr 24;27(19):2716-27. doi: 10.1038/sj.onc.1210934. Epub 2007 Nov 26.