Cai Zhejun, Li Fei, Peng Chuanmei, Li He, Zong Yiqiang, Liu Zhiguo, Qu Shen
Department of Biochemistry and Molecular Biology, Huazhong University of Science and Technology, Wuhan, 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2010 Oct;30(5):551-5. doi: 10.1007/s11596-010-0541-2. Epub 2010 Nov 10.
This study examined the effect of insulin on the expression of very low density lipoprotein receptor (VLDLR) subtypes of SGC7901 cells and discussed its biological implication. In vitro, moderately or poorly-differentiated human gastric adenocarcinoma cell line SGC7901 was incubated with insulin for different lengths of time, and then the expression of protein and RNA level in VLDLR subtypes were detected by Western blotting and real-time PCR, respectively. The results showed that, at certain time interval, insulin could down-regulate expression of type I VLDLR and up-regulate the expression of type II VLDLR in SGC7901 cells, at both protein and RNA level. We are led to conclude that insulin serves as a regulator in maintaining the balance between glucose and lipid metabolism in vivo, possibly through its effect on the differential expression of VLDLR subtypes.
本研究检测了胰岛素对SGC7901细胞极低密度脂蛋白受体（VLDLR）亚型表达的影响，并探讨其生物学意义。体外实验中，将中度或低分化的人胃腺癌细胞系SGC7901与胰岛素孵育不同时长，然后分别通过蛋白质印迹法和实时荧光定量PCR检测VLDLR亚型中蛋白质和RNA水平的表达。结果显示，在特定时间间隔内，胰岛素可在蛋白质和RNA水平下调SGC7901细胞中I型VLDLR的表达，并上调II型VLDLR的表达。由此我们得出结论，胰岛素可能通过影响VLDLR亚型的差异表达，在体内维持葡萄糖和脂质代谢平衡中发挥调节作用。
