Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, Shenyang, 110001, People's Republic of China.
J Gastrointest Surg. 2011 Jan;15(1):63-70. doi: 10.1007/s11605-010-1367-8. Epub 2010 Nov 10.
MicroRNAs (miRNAs) are small, non-coding RNAs of endogenous origin, they have been increasingly shown to have aberrant expression in many tumor types. miR-203 has not been comprehensively investigated in gastric and colorectal cancers.
Total RNA was extracted from tissues of 212 patients with gastric or colorectal cancer as well as from seven gastric and colorectal cell lines. We determined the expression of miR-203 by real-time PCR and calculated using the 2-ΔΔCt method. Then, we assessed miR-203 expression and clinicopathologic characteristics. Finally, we studied the effect of miR-203 on cell proliferation in SGC-7901 cells by MTT.
miR-203 has significantly low expression in colorectal cancer tissues (p < 0.001, paired t test) and cancer cell lines compared to non-tumor counterparts. Moreover, low expression of miR-203 was correlated with tumor size (p = 0.015, non-parametric test) and pT stage (p = 0.005) in colorectal cancer. Although expression of miR-203 was not significant in gastric cancer tissues (p = 0.124), interestingly, miR-203 was correlated with tumor size (p = 0.023), macroscopic type (p = 0.045), and pT stage (p = 0.013). Furthermore, we found miR-203 can inhibit the cell proliferation in SGC-7901 cells.
miR-203 may be related to the proliferation and invasion of gastric and colorectal cancers.
微小 RNA（miRNAs）是内源性起源的小非编码 RNA，它们在许多肿瘤类型中表达异常已被越来越多地证明。miR-203 在胃癌和结直肠癌中尚未得到全面研究。
从 212 例胃癌和结直肠癌患者的组织以及 7 株胃癌和结直肠癌细胞系中提取总 RNA。我们通过实时 PCR 测定 miR-203 的表达，并使用 2-ΔΔCt 法计算。然后，我们评估了 miR-203 表达与临床病理特征之间的关系。最后，我们通过 MTT 研究了 miR-203 对 SGC-7901 细胞增殖的影响。
miR-203 在结直肠癌组织（p<0.001，配对 t 检验）和癌细胞系中的表达明显低于非肿瘤对照。此外，miR-203 的低表达与结直肠癌的肿瘤大小（p=0.015，非参数检验）和 pT 分期（p=0.005）相关。尽管 miR-203 在胃癌组织中的表达不显著（p=0.124），但有趣的是，miR-203 与肿瘤大小（p=0.023）、大体类型（p=0.045）和 pT 分期（p=0.013）相关。此外，我们发现 miR-203 可以抑制 SGC-7901 细胞的增殖。
miR-203 可能与胃癌和结直肠癌的增殖和侵袭有关。
