Ohno I, Tanno Y, Yamauchi K, Takishima T
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Immunology. 1990 Feb;69(2):312-5.
P815, a transformed mouse mastocytoma cell line, produced and released cytotoxic factors after stimulation with phorbol 12-myristate 13-acetate (PMA), but not with lipopolysaccharide (LPS), calcium ionophore A23187 and IgE receptor triggering. The cytotoxic activity was reduced 60% by antibodies to mouse tumour necrosis factor (TNF). In addition, we demonstrated that TNF mRNA was already expressed under normal culture conditions and that it increased after stimulation with PMA. Although it was unknown whether factors other than TNF were lymphotoxin or some other unknown factors, it has been suggested that mast cells have cytotoxic activity, and that they contribute to inflammatory response through the release of TNF, which has a wide range of biological activity.
P815是一种转化的小鼠肥大细胞瘤细胞系，在用佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）刺激后会产生并释放细胞毒性因子，但用脂多糖（LPS）、钙离子载体A23187和IgE受体触发剂刺激则不会。抗小鼠肿瘤坏死因子（TNF）抗体可使细胞毒性活性降低60%。此外，我们证明TNF mRNA在正常培养条件下就已表达，并且在用PMA刺激后会增加。尽管除TNF之外的因子是淋巴毒素还是其他未知因子尚不清楚，但有研究表明肥大细胞具有细胞毒性活性，并且它们通过释放具有广泛生物活性的TNF来促进炎症反应。
