白细胞介素2（IL-2）诱导的白细胞介素2受体p75的酪氨酸磷酸化。
Interleukin 2 (IL-2)-induced tyrosine phosphorylation of IL-2 receptor p75.
作者信息
Asao H, Takeshita T, Nakamura M, Nagata K, Sugamura K
机构信息
Department of Microbiology, Tohoku University School of Medicine, Sendai, Japan.
出版信息
J Exp Med. 1990 Mar 1;171(3):637-44. doi: 10.1084/jem.171.3.637.
Abstract
We have recently established a mAb named TU11 mAb specific for the p75 subunit of human IL-2 receptor (IL-2R). The present study using TU11 mAb demonstrates the IL-2-induced phosphorylation of IL-2Rp75 on tyrosine residues in IL-2-dependent T cells. The tyrosine phosphorylation is mediated by the high affinity IL-2R, correlates with the IL-2-induced cell growth, and rapidly increases during the first 5 min of IL-2 stimulation. Phosphorylation of serine and threonine residues of IL-2Rp75 is also detected, but its IL-2 dependency is not significant during at least the first 5 min. These results suggest some roles of a tyrosine kinase associated with IL-2Rp75 in the IL-2-induced signal-transducing pathway.
摘要
我们最近制备了一种名为TU11单克隆抗体（mAb），它对人白细胞介素2受体（IL-2R）的p75亚基具有特异性。本研究使用TU11单克隆抗体证明了在IL-2依赖的T细胞中，IL-2可诱导IL-2Rp75的酪氨酸残基发生磷酸化。酪氨酸磷酸化由高亲和力IL-2R介导，与IL-2诱导的细胞生长相关，并在IL-2刺激的最初5分钟内迅速增加。还检测到IL-2Rp75丝氨酸和苏氨酸残基的磷酸化，但至少在最初5分钟内其对IL-2的依赖性不显著。这些结果表明与IL-2Rp75相关的酪氨酸激酶在IL-2诱导的信号转导途径中发挥了一些作用。
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本文引用的文献
1
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Mol Biol Med. 1984 Dec;2(6):379-96.
2
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Methods Enzymol. 1983;99:387-402. doi: 10.1016/0076-6879(83)99075-4.
3
Molecular cloning of cDNA encoding human interleukin-2 receptor.编码人白细胞介素-2受体的cDNA的分子克隆
Nature. 1984;311(5987):631-5. doi: 10.1038/311631a0.
4
Molecular cloning and expression of cDNAs for the human interleukin-2 receptor.人白细胞介素-2受体cDNA的分子克隆与表达
Nature. 1984;311(5987):626-31. doi: 10.1038/311626a0.
5
Characterization of interleukin 2-stimulated phosphorylation of 67 and 63 kDa proteins in human T-cells.人T细胞中白细胞介素2刺激的67 kDa和63 kDa蛋白磷酸化的特征分析
Biochem J. 1987 Feb 15;242(1):211-9. doi: 10.1042/bj2420211.
6
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J Biol Chem. 1988 May 25;263(15):6956-9.
7
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Microbiol Immunol. 1988;32(8):857-67. doi: 10.1111/j.1348-0421.1988.tb01447.x.
8
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9
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10
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