Lasekan J B, Clayton M K, Gendron-Fitzpatrick A, Ney D M
Department of Nutritional Sciences, University of Wisconsin-Madison 53706.
Nutr Cancer. 1990;13(3):153-63. doi: 10.1080/01635589009514056.
Interpretation of studies comparing the efficacy of different dietary fat sources in promoting 7,12-dimethylbenz[a]-anthracene (DMBA)-induced rat mammary tumorigenesis often ignores the fact that about 4% (wt/wt) linoleic acid (18:2n-6) is required for optimal tumor promotion. We therefore fed DMBA-intubated or placebo-intubated female, Sprague-Dawley rats 20% fat diets containing 18:2n-6 (wt/wt) from either high-linoleic safflower oil (SL, 14.6% 18:2n-6), high-oleic safflower oil (SO, 3.4% 18:2n-6), olive oil (OO, 1.1% 18:2n-6), or OO supplemented with 18:2n-6 (OL, 3.4% 18:2n-6) for 16 weeks. Results indicated that OO-fed rats had longer tumor-free time, fewer tumors per rat, and lower tumor incidence compared with SO and OL. Addition of 2.3% 18:2n-6 to OO enhanced tumor promotion (p less than 0.04); SL, SO, and OL demonstrated similar tumor-enhancement effect. About 74% of observed mammary tumors were adenocarcinomas; a greater number of tumors appeared in the thoracic and inguinal than in the cervical and abdominal regions irrespective of diet. These results indicate that once an optimal amount of linoleic acid is provided in the diet, oleic- or linoleic-rich oils have similar effects on promotion of mammary tumors in the rat.
比较不同膳食脂肪来源在促进7,12 - 二甲基苯并[a]蒽（DMBA）诱导的大鼠乳腺肿瘤发生方面功效的研究解读，常常忽略了一个事实，即最佳肿瘤促进需要约4%（重量/重量）的亚油酸（18:2n - 6）。因此，我们给经DMBA插管或安慰剂插管的雌性Sprague - Dawley大鼠喂食含20%脂肪的饮食，这些脂肪饮食中的亚油酸（18:2n - 6，重量/重量）分别来自高亚油酸红花油（SL，14.6% 18:2n - 6）、高油酸红花油（SO，3.4% 18:2n - 6）、橄榄油（OO，1.1% 18:2n - 6）或添加了18:2n - 6的橄榄油（OL，3.4% 18:2n - 6），持续16周。结果表明，与SO和OL相比，喂食OO的大鼠无瘤时间更长，每只大鼠的肿瘤数量更少，肿瘤发生率更低。向OO中添加2.3%的18:2n - 6增强了肿瘤促进作用（p小于0.04）；SL、SO和OL表现出相似的肿瘤增强效果。观察到的乳腺肿瘤约74%为腺癌；无论饮食如何，胸部和腹股沟出现的肿瘤数量多于颈部和腹部区域。这些结果表明，一旦饮食中提供了最佳量的亚油酸，富含油酸或亚油酸的油对大鼠乳腺肿瘤促进具有相似的作用。
