Argün Mahmut, Öner Mithat, Güney Ahmet, Halıcı Mehmet, Temizyürek Oğuz, Canöz Özlem
Department of Orthopedics and Traumatology, Erciyes University, Kayseri, Turkey.
Eklem Hastalik Cerrahisi. 2010 Dec;21(3):147-52.
This experimental study aims to compare the effects of three techniques; free autogenous periosteal graft, demineralized bone matrix (DBM) and basic fibroblast growth factor (bFGF) combined with periosteal graft on the healing of full thickness joint cartilage defects in rabbits.
This study used 87 adolescent 16 week-old New Zealand rabbits of both sexes, with an approximate weight of 2500-3750 g. The rabbits were randomly divided into four groups including a control group and three experimental groups. Cartilage defects were created in the posterior weight-bearing area of the medial femoral condyles of the rabbits. The surfaces of the osteochondral defects were covered with free autogenous periosteal graft, DBM and periosteal graft combined with bFGF in the experimental groups respectively. The rabbits were sacrificed at 4, 8 and 12 weeks postoperatively. Macroscopic and microscopic evaluations were performed.
Periosteal grafts and DBM respond to the repair of cartilage defects in varying degrees. Although the macroscopic evaluation scores were higher in the bFGF group, there was no statistically significant difference between groups (p>0.05). The total scores on the histological grading scale were significantly higher in the bFGF group and control group than the other groups at 4(th) and 8(th) weeks (p<0.05). At the 12(th) week the total score was significantly higher in the bFGF group than the other three groups (p<0.05).
The application of bFGF promoted regeneration of articular cartilage and led to successful cartilaginous resurfacing of defects within 12 weeks. We suggest that bFGF when combined with periosteal grafts may have excellent repair capacity in the restoration of osteochondral defects.
本实验研究旨在比较三种技术，即自体游离骨膜移植、脱矿骨基质（DBM）以及碱性成纤维细胞生长因子（bFGF）联合骨膜移植对兔全层关节软骨缺损愈合的影响。
本研究使用了87只16周龄的青春期新西兰兔，雌雄不限，体重约2500 - 3750克。将兔子随机分为四组，包括一个对照组和三个实验组。在兔内侧股骨髁的后负重区制造软骨缺损。实验组分别用自体游离骨膜移植、DBM以及bFGF联合骨膜移植覆盖骨软骨缺损表面。术后4周、8周和12周处死兔子，进行宏观和微观评估。
骨膜移植和DBM对软骨缺损的修复有不同程度的反应。虽然bFGF组的宏观评估分数较高，但各组之间无统计学显著差异（p>0.05）。在第4周和第8周时，bFGF组和对照组的组织学分级量表总分显著高于其他组（p<0.05）。在第12周时，bFGF组的总分显著高于其他三组（p<0.05）。
bFGF的应用促进了关节软骨的再生，并在12周内成功实现了缺损的软骨表面修复。我们认为，bFGF与骨膜移植联合应用时，在修复骨软骨缺损方面可能具有出色的修复能力。
