Department of Bioengineering and Pharmaceutics, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China.
J Chromatogr A. 2010 Dec 10;1217(50):7833-9. doi: 10.1016/j.chroma.2010.10.079. Epub 2010 Oct 23.
Evodiamine and rutaecarpine have been intensively studied due to their pharmacological actions and clinical applications. In this report, supercritical fluid was used to extract evodiamine and rutaecarpine from the unripe fruit of Evodia rutaecarpa. Response surface methodology using Box-Behnken experimental design was utilized to optimize parameters for supercritical carbon dioxide extraction with methanol as co-solvent. The effect of various values of dynamic extraction time (30-90min), temperature (50-70°C) and pressure (200-400bar) on extraction yields of the two compounds was evaluated. Determinations of the extracts were performed by high-performance liquid chromatography. The experimental data obtained were fitted to second-order polynomial equations and analyzed by analysis of variance. The highest yields predicted were 1.217mg/g for evodiamine and 0.969mg/g for rutaecarpine at the optimal values (time 78min, temperature 62°C, pressure 280bar and co-solvent flow rate 0.4mL/min), based on the selected range of experimental conditions.
由于其药理作用和临床应用，吴茱萸碱和吴茱萸卡品碱受到了广泛的研究。在本报告中，使用超临界流体从吴茱萸未成熟的果实中提取吴茱萸碱和吴茱萸卡品碱。采用 Box-Behnken 实验设计的响应面法，优化了超临界二氧化碳萃取的参数，甲醇作为共溶剂。评估了动态萃取时间（30-90min）、温度（50-70°C）和压力（200-400bar）的各种值对两种化合物萃取率的影响。通过高效液相色谱法对提取物进行测定。根据所选的实验条件范围，用方差分析对实验数据进行二次多项式方程拟合和分析。预测的最高产率为吴茱萸碱 1.217mg/g，吴茱萸卡品碱 0.969mg/g，最佳值为（时间 78min、温度 62°C、压力 280bar 和共溶剂流速 0.4mL/min）。
