Ahmadpour Roghayeh, Zanjani Bahram Maleki, Garoosi Ghasem-Ali, Farjaminezhad Reza, Haddad Raheem
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran.
Heliyon. 2024 Oct 5;10(19):e38856. doi: 10.1016/j.heliyon.2024.e38856. eCollection 2024 Oct 15.
is an important medicinal plant used in medicine and contains tropane alkaloid compounds such as hyoscyamine and scopolamine. In this study, after the selection of the solvent for extracting hyoscyamine and scopolamine, the central composite design of the response surface methodology was used to study the effect of solvent concentrations (0, 25, 50, 75, and 100 %), temperatures (25, 30, 35, 40, and 45 °C) and ultrasonication times (10, 20, 30, 40, and 50 min). The hyoscyamine and scopolamine content were obtained by HPLC-DAD. The results indicated that the predicted optimal condition for hyoscyamine and scopolamine extraction from root was as follows. Hyoscyamine: 100 % methanol, temperature 45 °C and ultrasonication time 10 min, obtained 172.06 μg/g dry weight; and scopolamine: 98.50 % methanol, temperature 25 °C and ultrasonication time 10 min, provided 229.48 μg/g dry weight. To confirm the predicted extraction conditions, a separate experiment was conducted, and the results showed that the hyoscyamine and scopolamine contents were 164.72 and 209.23 μg/g dry weight, respectively.
是一种重要的药用植物，用于医学领域，含有托烷生物碱类化合物，如莨菪碱和东莨菪碱。在本研究中，在选择用于提取莨菪碱和东莨菪碱的溶剂后，采用响应面法的中心复合设计来研究溶剂浓度（0、25、50、75和100%）、温度（25、30、35、40和45℃）和超声处理时间（10、20、30、40和50分钟）的影响。通过高效液相色谱 - 二极管阵列检测器（HPLC - DAD）测定莨菪碱和东莨菪碱的含量。结果表明，从根中提取莨菪碱和东莨菪碱的预测最佳条件如下。莨菪碱：100%甲醇、温度45℃、超声处理时间10分钟，获得172.06μg/g干重；东莨菪碱：98.50%甲醇、温度25℃、超声处理时间10分钟，得到229.48μg/g干重。为了验证预测的提取条件，进行了单独的实验，结果表明莨菪碱和东莨菪碱的含量分别为164.72和209.23μg/g干重。
