Huang Zhifang, Yi Jinhai, Wu Yan, Liu Yunhua, Chen Yan, Liu Yuhong
Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China.
Zhongguo Zhong Yao Za Zhi. 2011 Feb;36(4):478-80.
A HPLC method for determination of limonin, evodiamine and rutaecarpine in Evodia rutaecarpa was optimized. The mobile phase was [acetonitrile-tetrahydrofuran (25: 15)] -0.02% H3 PO4 (35:65). The detection wavelength was 220 nm and the flow rate was 1.0 mL x min(-1). Limonin, evodiamine and rutaecarpine were all well separated from other substances and their UV spectrums were essentially the same to the standards . The liner ranges of limonin, evodiamine and rutaecarpine were 0.196 8-3.936, 0.153 6-3.072, 0.097 4-1.948 microg. The average recoveries were 97.8%, 100.7% and 98.4%. RSD were 1.7%, 1.3% and 1.1% (n = 6). The method of this article is accurate, reproducible and can be used to enhance the quality control of E. rutaecarpa.
优化了一种用于测定吴茱萸中柠檬苦素、吴茱萸碱和吴茱萸次碱的高效液相色谱法。流动相为[乙腈 - 四氢呋喃（25:15）]-0.02% H3PO4（35:65）。检测波长为220 nm，流速为1.0 mL·min(-1)。柠檬苦素、吴茱萸碱和吴茱萸次碱均与其他物质得到良好分离，且其紫外光谱与标准品基本一致。柠檬苦素、吴茱萸碱和吴茱萸次碱的线性范围分别为0.196 8 - 3.936、0.153 6 - 3.072、0.097 4 - 1.948 μg。平均回收率分别为97.8%、100.7%和98.4%。相对标准偏差分别为1.7%、1.3%和1.1%（n = 6）。本文方法准确、可重现，可用于加强吴茱萸的质量控制。
