[吴茱萸高效液相色谱测定方法的优化]

[Optimization of a HPLC determination method for Evodia rutaecarpa].

作者信息

Huang Zhifang, Yi Jinhai, Wu Yan, Liu Yunhua, Chen Yan, Liu Yuhong

机构信息

Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2011 Feb;36(4):478-80.

PMID:21598546

Abstract

A HPLC method for determination of limonin, evodiamine and rutaecarpine in Evodia rutaecarpa was optimized. The mobile phase was [acetonitrile-tetrahydrofuran (25: 15)] -0.02% H3 PO4 (35:65). The detection wavelength was 220 nm and the flow rate was 1.0 mL x min(-1). Limonin, evodiamine and rutaecarpine were all well separated from other substances and their UV spectrums were essentially the same to the standards . The liner ranges of limonin, evodiamine and rutaecarpine were 0.196 8-3.936, 0.153 6-3.072, 0.097 4-1.948 microg. The average recoveries were 97.8%, 100.7% and 98.4%. RSD were 1.7%, 1.3% and 1.1% (n = 6). The method of this article is accurate, reproducible and can be used to enhance the quality control of E. rutaecarpa.

摘要

优化了一种用于测定吴茱萸中柠檬苦素、吴茱萸碱和吴茱萸次碱的高效液相色谱法。流动相为[乙腈 - 四氢呋喃（25:15）]-0.02% H3PO4（35:65）。检测波长为220 nm，流速为1.0 mL·min(-1)。柠檬苦素、吴茱萸碱和吴茱萸次碱均与其他物质得到良好分离，且其紫外光谱与标准品基本一致。柠檬苦素、吴茱萸碱和吴茱萸次碱的线性范围分别为0.196 8 - 3.936、0.153 6 - 3.072、0.097 4 - 1.948 μg。平均回收率分别为97.8%、100.7%和98.4%。相对标准偏差分别为1.7%、1.3%和1.1%（n = 6）。本文方法准确、可重现，可用于加强吴茱萸的质量控制。