Department of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907-2054, USA.
J Virol. 2011 Jan;85(2):835-41. doi: 10.1128/JVI.01847-10. Epub 2010 Nov 10.
Hantaan virus is the prototypic member of the Hantavirus genus within the family Bunyaviridae and is a causative agent of the potentially fatal hemorrhagic fever with renal syndrome. The Bunyaviridae are a family of negative-sense RNA viruses with three-part segmented genomes. Virions are enveloped and decorated with spikes derived from a pair of glycoproteins (Gn and Gc). Here, we present cryo-electron tomography and single-particle cryo-electron microscopy studies of Hantaan virus virions. We have determined the structure of the tetrameric Gn-Gc spike complex to a resolution of 2.5 nm and show that spikes are ordered in lattices on the virion surface. Large cytoplasmic extensions associated with each Gn-Gc spike also form a lattice on the inner surface of the viral membrane. Rod-shaped ribonucleoprotein complexes are arranged into nearly parallel pairs and triplets within virions. Our results differ from the T=12 icosahedral organization found for some bunyaviruses. However, a comparison of our results with the previous tomographic studies of the nonpathogenic Tula hantavirus indicates a common structural organization for hantaviruses.
汉坦病毒是布尼亚病毒科汉坦病毒属的原型成员，是一种可能致命的肾综合征出血热的病原体。布尼亚病毒科是一类具有三段节段负义 RNA 基因组的病毒。病毒粒子被带有来自一对糖蛋白（Gn 和 Gc）的刺突的包膜所装饰。在这里，我们展示了汉坦病毒病毒粒子的低温电子断层扫描和单颗粒低温电子显微镜研究。我们已经确定了四聚体 Gn-Gc 刺突复合物的结构，分辨率为 2.5nm，并表明刺突在病毒粒子表面的晶格中有序排列。与每个 Gn-Gc 刺突相关的大细胞质延伸也在病毒膜的内表面形成晶格。杆状核糖核蛋白复合物在病毒粒子内排列成近乎平行的对和三胞胎。我们的结果与一些布尼亚病毒的 T=12 二十面体组织不同。然而，将我们的结果与以前对非致病性图拉汉坦病毒的层析研究进行比较表明，汉坦病毒具有共同的结构组织。
