Kadyrova A A, Fomina A N, Ershov F I, Novokhatskiĭ A S
Antibiotiki. 1978 Aug;23(8):741-7.
Biological activity of a new natural interferon inductor, the replicative RNA form of phage f2 (RFf2) was studied. A possibility of using RFf2 for production of highly active interferon under conditions of superinduction providing an increase in the interferon yield by to 256--512 times as compared to the control samples was shown. The protective interferonogenic and antiviral effect of RFf2 in mice infected with Semliki forest virus (SFV) and tickborne encephalitis virus (TBEV) was studied on administration of the inductor by various routes. It was found that intraperitoneal administration of RFf2 in a dose of 10 gamma per a mouse protected the infected animals from death. It was accompanied by production of up to 1280 units/ml of interferon in the blood serum of the animals. Maximum protection of the animals from death under conditions of the experiment (80 per cent on infection with SFV and 65 per cent on infection with TBEV) was observed when the preparation was administered twice: 4 hours after the infection. Combined use of RFf2 with chemotherapeutics (rimantadine) increased the protective effect both in the tissue culture and in vivo.
研究了一种新型天然干扰素诱导剂——噬菌体f2的复制RNA形式（RFf2）的生物活性。结果表明，在超诱导条件下使用RFf2生产高活性干扰素是可行的，与对照样品相比，干扰素产量可提高256至512倍。通过不同途径给予诱导剂，研究了RFf2对感染Semliki森林病毒（SFV）和蜱传脑炎病毒（TBEV）的小鼠的保护性干扰素生成和抗病毒作用。发现以每只小鼠10微克的剂量腹腔注射RFf2可保护感染动物免于死亡。这伴随着动物血清中产生高达1280单位/毫升的干扰素。当制剂在感染后4小时给药两次时，在实验条件下观察到动物免于死亡的最大保护率（感染SFV时为80%，感染TBEV时为65%）。RFf2与化学治疗剂（金刚乙胺）联合使用可增强组织培养和体内的保护作用。
