Bukata L A, Nosik N N, Fomina A N, Ershov F I
Antibiotiki. 1979 Jun;24(6):444-8.
Biological activities of the RNA replicative form of phage f2, a natural interferon inductor and poly-I -- poly-C, a synthetic polyribonucleotide complex were studied comparatively. Differences in the comparative interferonogenic and antiviral activity of the inductors were as dependent on the type of the cell system. It was shown that DEAE-dextran increased the interferon-inducing activity of RFf2 in the cell culture by 4 to 8 times. The dynamics of the interferonogenic and antiviral activity of RFf2 in the L-929 cell culture was studied. Interferon appeared in the culture fluid in 6--8 hours and reached its maximum titers (128 IU50/ml) by the 24th hour, the maximum protection of the cells being also developed by the 12th--24th hour, reaching on an average 51 g PFU/ml. It was shown in the experiments with green marmosets that administration of RFf2 in the form of aerosol in a dose of 2.3 mg/kg induced interferon production in the blood serum the titers of which amounted to 80--160 IU50/ml 24 hours after the administration.
对噬菌体f2的RNA复制形式（一种天然干扰素诱导剂）和聚肌苷酸-聚胞苷酸（一种合成多聚核糖核苷酸复合物）的生物活性进行了比较研究。诱导剂在比较干扰素生成和抗病毒活性方面的差异取决于细胞系统的类型。结果表明，二乙氨基乙基葡聚糖可使细胞培养物中RFf2的干扰素诱导活性提高4至8倍。研究了RFf2在L-929细胞培养物中的干扰素生成和抗病毒活性动态。干扰素在6至8小时出现在培养液中，到第24小时达到最高滴度（128 IU50/ml），细胞的最大保护作用也在第12至24小时形成，平均达到51 g PFU/ml。在绿狨猴实验中表明，以2.3 mg/kg剂量气溶胶形式给予RFf2可诱导血清中产生干扰素，给药后24小时其滴度达80至160 IU50/ml。
