University of Piemonte Orientale, DISAV - Dipartimento di Scienze dell'Ambiente e della Vita, viale T. Michel 11, 15121 Alessandria, Italy.
J Chromatogr A. 2010 Dec 10;1217(50):7864-72. doi: 10.1016/j.chroma.2010.10.049. Epub 2010 Oct 23.
A rapid on-line solid phase extraction ultra high performance liquid chromatography tandem mass spectrometry method was developed for the identification and quantitation of nine perfluorinated compounds in matrices of environmental, biological and food interest. Pre-treatment, solid phase extraction, chromatographic and mass detection conditions were optimised, in order to apply the whole methodology to the analysis of different matrices. Particular attention was devoted to the evaluation of matrix effect and the correlated phenomena of ion enhancement or suppression in mass spectrometry detection. LOD and LOQ range from 3 to 15ngL(-1) and from 10 to 50ngL(-1), respectively. Method detection limits (MDLs) were also calculated for each kind of matrix. The recovery, evaluated for each analyte, does not depend on analyte concentration in the explored concentration range: average R¯% values are always greater than 82.9%. In the whole, the results obtained for samples of river waters, blood serum, blood plasma, and fish confirm the ubiquitous presence of perfluorinated compounds, as recently denounced by many sources.
建立了一种快速在线固相萃取超高效液相色谱-串联质谱法，用于鉴定和定量环境、生物和食品感兴趣基质中的 9 种全氟化合物。优化了预处理、固相萃取、色谱和质谱检测条件，以便将整个方法应用于不同基质的分析。特别关注了基质效应的评估以及质谱检测中离子增强或抑制的相关现象。LOD 和 LOQ 的范围分别为 3 至 15ng/L 和 10 至 50ng/L。还为每种基质计算了方法检测限（MDL）。对于每种分析物，评估的回收率不依赖于所研究浓度范围内的分析物浓度：平均 R¯%值始终大于 82.9%。总的来说，从河流、血清、血浆和鱼类样本中获得的结果证实了全氟化合物的普遍存在，这是最近许多来源所谴责的。
