Monika Kujawa-Hadryś, Dariusz Tosik, Hieronim Bartel
Department of Histology and Tissue Ultrastructure, Łódż Medical University, Łódż, Poland.
Folia Histochem Cytobiol. 2010 Sep 30;48(3):371-6. doi: 10.2478/v10042-010-0043-x.
Caffeine is one of the most frequently consumed psychoactive substances. It has been known for many years that caffeine at high concentrations exerts harmful effects on both women's and laboratory animals' fertility, moreover it may impair normal development of many organs in the prenatal period. So far there have been few studies performed that demonstrate teratogenic effects of caffeine on structures of the developing eye, particularly the cornea. The aim of the study was to show ultrastructural changes in the developing cornea, as the effect of caffeine administration to chicken embryos. The experimental materials were 26 chicken embryos from incubated breeding eggs. Eggs were divided into two groups: control (n=30) in which Ringer liquid was administrated, and experimental (n=30) in which teratogenic dose of caffeine 3.5mg/egg was given. In 36th hour of incubation solutions were given with cannula through hole in an egg shell directly onto amniotic membrane. After closing the hole with a glass plate and paraffine, eggs were put back to incubator. In 10th and 19th day of incubation corneas were taken for morphological analysis with a use of electron microscopy. Administration of caffeine during chicken development causes changes of collagen fibers of Bowman's membrane patterns and of the corneal stroma but it also changes proportion of amount of collagen fibers and of the stromal cells.
咖啡因是最常摄入的精神活性物质之一。多年来人们已经知道，高浓度的咖啡因会对女性和实验动物的生育能力产生有害影响，此外，它还可能损害许多器官在孕期的正常发育。到目前为止，很少有研究表明咖啡因对发育中的眼睛结构，特别是角膜有致畸作用。本研究的目的是展示给鸡胚施用咖啡因后发育中角膜的超微结构变化。实验材料是来自孵化种蛋的26个鸡胚。将蛋分为两组：对照组（n = 30），给予林格液；实验组（n = 30），给予致畸剂量的咖啡因3.5mg/蛋。在孵化的第36小时，通过蛋壳上的孔用套管将溶液直接滴加到羊膜上。用玻璃板和石蜡封闭孔后，将蛋放回孵化器。在孵化的第10天和第19天，取角膜用于电子显微镜形态学分析。在鸡发育过程中施用咖啡因会导致Bowman膜模式和角膜基质的胶原纤维发生变化，但也会改变胶原纤维和基质细胞数量的比例。
